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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-20029 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Bcl-G Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is U937 cell lysate. PA5-20029 can be used with blocking peptide PEP-0148.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Inhibition of histone methyltransferase G9a attenuates liver cancer initiation by sensitizing DNA-damaged hepatocytes to p53-induced apoptosis.
Nakatsuka T, Tateishi K, Kato H, Fujiwara H, Yamamoto K, Kudo Y, Nakagawa H, Tanaka Y, Ijichi H, Ikenoue T, Ishizawa T, Hasegawa K, Tachibana M, Shinkai Y, Koike K
Cell death & disease 2021 Jan 19;12(1):99
Cell death & disease 2021 Jan 19;12(1):99
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of U937 cell lysates using a Bcl-G polyclonal antibody (Product # PA5-20029) at (A) 2.5 and (B) 5 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of Bcl-G in U937 cell lysates with Bcl-G Polyclonal Antibody (Product # PA5-20029) at (A) 2.5 and (B) 5 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of mouse testis cells using a Bcl-G polyclonal antibody (Product # PA5-20029) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of Bcl-G in Mouse Testis cells with Bcl-G Polyclonal Antibody (Product # PA5-20029) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of mouse testis tissue using Bcl-G Polyclonal Antibody (Product # PA5-20029) at 2 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 G9a protects hepatocytes against apoptosis after a high dose of DEN. A Immunohistochemical analysis of the mice liver 48 h after administering a high dose of diethylnitrosamine (DEN) (100 mg/kg i.p.). G9a is upregulated after DEN administration, and cleaved-Caspase 3 induction occurs only in the G9a DeltaHep liver, whereas gammaH2AX and p53 levels are comparable between wild-type (WT) and G9a DeltaHep livers. Bcl-G expression is elevated in the pericentral hepatocytes of G9a DeltaHep mice. Scale bars, 50 um. The number of positive hepatocytes is shown in the graph below (** p < 0.01, NS not significant, Student's t test). B Serum ALT levels are significantly elevated in G9a DeltaHep mice 48 h after administering a high dose of DEN. Data are represented as mean +- SEM (WT/DEN0h, n = 4; G9a DeltaHep /DEN0h, n = 3; WT/DEN48h, n = 5; G9a DeltaHep /DEN48h, n = 3). C Cyp2e1 expression, as assessed by qRT-PCR, is comparable between WT and G9a DeltaHep livers (each, n = 3; NS not significant, Student's t test). D Top 10 upregulated genes in the non-tumorous area of G9a DeltaHep liver are listed. E qRT-PCR analysis validates Bcl-G upregulation in the G9a DeltaHep liver (* p < 0.05, Student's t test). F Immunoblots reveal increased Bcl-G, cleaved-PARP, and cleaved-Caspase3 protein levels in the G9a DeltaHep liver 48 h after high-dose DEN administration. Right graph shows relative protein level of Bcl-G (each, n = 3; ** p < 0.01, NS not significant, Student's t test). G Relative
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 4 BCL-G is involved in apoptosis during the DNA damage response in hepatocytes. A BCL-G overexpression induces apoptotic changes in normal human hepatocytes. Representative immunoblot images and relative expression levels of the proteins are shown (each, n = 3; * p < 0.05, Student's t test). B , D The G9a inhibitor UNC0638 (5 µM) promotes apoptosis in hepatocytes after treatment with hydrogen peroxide (H 2 O 2 , 2 mM) or UVB irradiation (300 J/m 2 ) with an increase in BCL-G expression. Bcl-G knockdown (KD) suppresses the excessive induction of apoptosis caused by a G9a inhibitor. Right graphs show quantification of relative protein levels. C , E Representative dot-plot diagrams showing the percentage of the hepatocyte lines stained with Annexin V-FITC and propidium iodide (PI) after 24 h of treatment with H 2 O 2 or UVB irradiation. The results of three independent experiments are shown in the right graph (** p < 0.01, NS not significant, Student's t test).
