- PA5-25035 - Invitrogen Antibodies BTG1 antibody

Submitted references Cystine and Methionine Deficiency Promotes Ferroptosis by Inducing B-Cell Translocation Gene 1.

Cho IJ, Kim D, Kim EO, Jegal KH, Kim JK, Park SM, Zhao R, Ki SH, Kim SC, Ku SK
Antioxidants (Basel, Switzerland) 2021 Sep 28;10(10)

B‑cell translocation gene 1 serves as a novel prognostic indicator of hepatocellular carcinoma.

Kanda M, Sugimoto H, Nomoto S, Oya H, Hibino S, Shimizu D, Takami H, Hashimoto R, Okamura Y, Yamada S, Fujii T, Nakayama G, Koike M, Fujiwara M, Kodera Y
International journal of oncology 2015 Feb;46(2):641-8

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis using a BTG1 polyclonal antibody (Product # PA5-25035) in mouse NIH-3T3 tissue lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis using a BTG1 polyclonal antibody (Product # PA5-25035) in MCF-7 cell lysates (35 µg per lane).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human colon carcinoma using a BTG1 polyclonal antibody (Product # PA5-25035), followed by HRP-conjugated secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 3 Cystine and methionine deficiency induces BTG1 by activating ATF4. HepG2 and L-O2 cells were exposed to CST/Met (-) for 1-12 ( a , d ), or 24 h ( b , c , e , f ). ( a ) The level of BTG1 mRNA was determined by qPCR. Fer-1 (10 muM, 12 h) or ISRIB (1 muM, 12 h) was simultaneously applied to HepG2 cells under CST/Met (-) (left). ( b ) BTG1 protein. Open arrowheads and dashed lines in immunoblot images indicate nonspecific bands and cropped images of the same membrane, respectively (upper). ( c ) BTG1 transactivation. Schematic illustration of constructed reporter plasmids containing the human and murine BTG1 gene promoter (upper). An ATF4 expression plasmid was co-transfected with hpBTG1-luc, mpBTG1-luc, or mpBTG1-DeltaA4RE-luc. pCDNA3.2/V5-DEST was used for mock transfection (lower). ( d - f ) Effect of siATF4 on BTG1 induction. A siRNA targeting human ATF4 ( d , e ) or murine ATF4 ( f ) was transfected into HepG2 cells, and expression of BTG1 ( d , e ) and transactivation ( f ) were determined by qPCR, immunoblot, and reporter gene assays, respectively. ** p < 0.01, * p < 0.05, versus control ( a , b , d , e ) or mock transfection ( c , f ); ## p < 0.01, # p < 0.05, versus HepG2 cells exposed to CST/Met (-) ( a , d , e ) or ATF4-transfected cells ( c , f ); ++ p < 0.01, between basal level of ATF4 mRNA ( d ): A4RE, putative ATF4 response element; Con, control.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 BTG1 induces ferroptosis under cystine and methionine deficiency. WT and BTG1 KO HAP1 cells were exposed to CST/Met (-) for 1 ( a --lower), 12 ( c , e ), 24 ( a --upper, and b , d ), or 0-24 h ( f ). ( a ) Fluorescence intensity was measured after incubating HAP1 cells with CST/Met (-) and DCFH-DA (lower). The phenotype of BTG1 KO HAP1 cells was verified by BTG1 immunoblotting. Open arrowheads and dashed lines in immunoblot images indicate nonspecific bands and cropped images of the same membrane, respectively (upper). ( b ) Effect of BTG1 KO on CST/Met (-)-inducible expression of ATF4. ( c ) mRNA levels of ISR target genes in HAP1 cells. ( d - f ) Lipid peroxidation ( d ), PTGS2 mRNA level ( e ), and necrotic cell death ( f ) in HAP1 cells exposed to CST/Met (-). ** p < 0.01, * p < 0.05, versus control; ## p < 0.01, between HAP1 cells exposed to CST/Met (-); N.S., not significant.

PA5-25035

Antibody data