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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA501277 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA501277, RRID:AB_11123819
- Product name
- Anti-ARNTL (BMAL1) mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)
- Antibody type
- Monoclonal
- Description
- Anti-ARNTL (BMAL1) mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- ARNTL
- Isotype
- IgG
- Antibody clone number
- OTI3G9
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
Submitted references Circadian Modulation of 8-Oxoguanine DNA Damage Repair.
Manzella N, Bracci M, Strafella E, Staffolani S, Ciarapica V, Copertaro A, Rapisarda V, Ledda C, Amati M, Valentino M, Tomasetti M, Stevens RG, Santarelli L
Scientific reports 2015 Sep 4;5:13752
Scientific reports 2015 Sep 4;5:13752
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ARNTL (RC207870, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ARNTL.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ARNTL monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-ARNTL monoclonal antibody.(1:200)
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-ARNTL mouse monoclonal antibody (TA501277) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ARNTL(RC207870).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207870 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-ARNTL antibody(TA501277), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-ARNTL antibody(TA501277),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
