- 27848-1-AP - Invitrogen Antibodies ABCC6 antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Mouse brain tissue were subjected to SDS PAGE followed by western blot with 27848-1-AP (ABCC6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 27848-1-AP (ABCC6 antibody) at dilution of 1:200 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded mouse liver tissue slide using 27848-1-AP (ABCC6 antibody) at dilution of 1:200 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: The clinical outcomes of ABCC6 in patients with HCC. (A) BioGPS database showing the mRNA expression level of ABCA6 , ABCC6 , and ABCG5 in different normal human tissues. (B) Western blotting displaying ABCC6 protein levels in 13 pairs of HCC tumor and paratumor tissues. (C) Statistical analysis showing the differential protein level of ABCC6 in Renji Hospital hepatectomy HCC tissue array. (D) Representative IHC figure showing the ABCC6 protein level in Renji Hospital hepatectomy HCC tissue array. (E) Representative IHC figure showing the ABCC6 protein level in Renji Hospital liver transplantation HCC tissue array. (F) Kaplan-Meier analysis showing the overall survival and disease-free survival of HCC patients in correlation with high or low ABCC6 levels in Renji Hospital hepatectomy cohort. (G) Kaplan-Meier analysis showing the overall survival and disease-free survival of HCC patients in correlation with high or low ABCC6 levels in Renji Hospital liver transplantation cohort. (H) Heatmap and histogram showing the expression of proliferation-related genes in correlation with different ABCC6 expression level. Data was derived from TCGA dataset. Error bars indicate means +- SEM. P -values were determined by two tailed t -test (C) , one-way ANOVA (H) and Kaplan-Meier analysis (F, G) . **** P < 0.0001.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 4 ABCC6 knockdown promotes HCC cell lines proliferation in vitro and in vivo . (A) qPCR analysis showing ABCC6 mRNA expression level in different HCC cell lines. (B) Western blot displaying ABCC6 protein level in different HCC cell lines as well as one normal human hepatic cell line (L-02). (C) Western Blot validating the ABCC6 knockdown efficiency in 97H and Huh7 cell lines. (D) Western Blot validating the ABCC6 overexpression efficiency in 7721 and LM3 cell lines. (E) CCK-8 assay showing the cell proliferation of ABCC6 knockdown 97H and Huh7 cells. (F) Colony formation assay of ABCC6 knockdown 97H cells. (G) Edu incorporation assay showing the effect of ABCC6 knockdown on the cell proliferative ability of 97H and Huh7 cells. (H) CCK-8 assay showing the cell proliferation of ABCC6 overexpression 7721 and LM3 cells. (I) Colony formation assay of ABCC6 overexpression 7721 cells. (J) Edu incorporation assay showing the effect of ABCC6 overexpression on the cell proliferative ability of 7721 and LM3 cells. (K) Images showing the subcutaneous xenograft of ShNS, ShABCC6-1, and ShABCC6-2 97H cells. n=4; The scale bar indicates 1cm. (L) The growth curves of ShNS, ShABCC6-1, and ShABCC6-2 97H cells subcutaneously injected into NSG immunodeficient mice. (M) The weight of tumors collected from xenograft NSG mouse model implanted with ShNS, ShABCC6-1, and ShABCC6-2 97H cells. Error bars indicate means +- SEM. P -values were determined by two tailed t -test (E-J) and one-way ANOVA

27848-1-AP

Antibody data