V2115

antibody from NSJ Bioreagents

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2115

Provider

NSJ Bioreagents

Product name

Estrogen Receptor beta Antibody

Antibody type

Monoclonal

Antigen

The C-terminus fragment of recombinant human Estrogen Receptor beta protein was used as the immunogen for this antibody.

Description

Protein G purified monoclonal antibody

Reactivity

Human, Mouse, Rat, Horse, Porcine, Simian

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

ERb455

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the Estrogen Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of breast cancer stained with Estrogen Receptor beta antibody (ERb455).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of ovarian cancer stained with Estrogen Receptor beta antibody (ERb455).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of gastric carcinoma stained with Estrogen Receptor beta antibody (ERb455).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of bladder carcinoma stained with Estrogen Receptor beta antibody (ERb455).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of MCF-7 cells: Black=cells alone; Green=isotype control; Red=Estrogen Receptor beta antibody PE conjugate

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of BT474 cells: Black=cells alone; Gray=isotype control; Green=Estrogen Receptor beta antibody AF488 conjugate

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE Analysis of Purified, BSA-Free Estrogen Receptor beta Antibody (clone ERb455). Confirmation of Integrity and Purity of the Antibody.