V8667

antibody from NSJ Bioreagents

Targeting: ESR2 ER-beta, Erb, NR3A2

Western blot (Data presented on provider website)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V8667

Provider

NSJ Bioreagents

Product name

Estrogen Receptor beta 1 Antibody

Antibody type

Monoclonal

Description

Purified

Reactivity

Human

Host

Mouse

Antibody clone number

PPG5/10

Vial size

20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the Estrogen Receptor beta 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human breast carcinoma with Estrogen Receptor beta 1 antibody (clone PPG5/10). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of PFA-fixed human MCF7 cells with Estrogen Receptor beta 1 antibody (clone PPG5/10); Red=isotype control, Blue= Estrogen Receptor beta 1 antibody.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free Estrogen Receptor beta 1 antibody (clone PPG5/10) as confirmation of integrity and purity.