25276-1-AP

antibody from Invitrogen Antibodies

Targeting: PHOX2B NBPhox, PMX2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

25276-1-AP

Provider

Invitrogen Antibodies

Product name

PHOX2B Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: GSCSLGTLR DHQSSPYAAV PYKLFTDHGG LNEKRKQRRI RTTFTSAQLK ELERVFAETH YPDIYTREEL ALKIDLTEAR VQVWFQNRRA KFRKQERAAA AAAAAAKNGS SGKKSDSSRD DESKEAKSTD PDSTGGPGPN PNPTPSCGAN GGGGGGPSPA GAPGAAGPGG PGGEPGKGGA AAAAAAAAAA AAAAAAAAAG GLAAAGGPGQ GWAPGPGPIT SIPDSLGGPF ASVLSSLQRP NGAKAALVKS SMF (63-314 aa encoded by BC017199)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.4 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 25276-1-AP (PHOX2B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neuro-2a cells were subjected to SDS PAGE followed by western blot with 25276-1-AP ( PHOX2B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Neuro-2a cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using 25276-1-AP (PHOX2B antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded mouse brain tissue slide using 25276-1-AP (PHOX2B Antibody) at dilution of 1:200 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded neuroblastoma tissue slide using 25276-1-AP ( PHOX2B Antibody) at dilution of 1:200 (under 40x lens).