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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunohistochemistry [1]
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- Product number
- MA3-814 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S-arrestin Monoclonal Antibody (PDS1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA3-814 detects Visual Arrestin from human, rat, porcine, bovine, ovine, and goat tissues.
- Antibody clone number
- PDS1
- Concentration
- Conc. Not Determined
Submitted references Analysis of antigenic determinants of retinal S-antigen with monoclonal antibodies.
Banga JP, LeRoy F, Suleyman S, Kasp E, Brown E, Dumonde D
Investigative ophthalmology & visual science 1988 Jan;29(1):12-21
Investigative ophthalmology & visual science 1988 Jan;29(1):12-21
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemistry was performed on human retina tissue. To expose target protein, antigen was retreived using 10mM sodium citrate followed by microwave treatment for 8-15 minutes. Endogenous peroxidases were blocked in 3% H202-methanol for 15 minutes and tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Visual Arrestin mouse monoclonal antibody (Product # MA3-814) at a dilution of 1:20 overnight in a humidified chamber. Tissues were washed in PBST and detection was performed using a secondary antibody conjugated to HRP. DAB staining buffer was applied and tissues were counterstained with hematoxylin and prepped for mounting. Images were taken at 40X magnification.
