MA5-38652

antibody from Invitrogen Antibodies

Targeting: TWIST1 ACS3, bHLHa38, BPES2, BPES3, CRS, CRS1, H-twist, SCS, TWIST

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38652

Provider

Invitrogen Antibodies

Product name

TWIST1 Monoclonal Antibody (10E4E6)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

This antibody has been tested in indirect ELISA.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

10E4E6

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TWIST1 using TWIST1 Monoclonal Antibody (Product # MA5-38652) in HEK293 (1) and TWIST1 (AA: 9-74)-human IgG-Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TWIST1 using TWIST1 Monoclonal Antibody (Product # MA5-38652) in NIH/3T3 (1), JURKAT (2), HeLa (3), A549 (4), RAJI (5) and OCM-1 (6) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis using TWIST1 Monoclonal Antibody (Product # MA5-38652) against human TWIST1 recombinant protein. (Expected MW is 31.9 kDa)

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of TWIST1 using TWIST1 Monoclonal Antibody (Product # MA5-38652) in HEK293 (1) and TWIST1 (AA: 9-74)-human IgG-Fc transfected HEK293 (2) cell lysate.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of TWIST1 in HeLa cells using TWIST1 Monoclonal Antibody (Product # MA5-38652) (green). Blue: DRAQ5 fluorescent DNA dye.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TWIST1 in paraffin-embedded cervical cancer tissues using TWIST1 Monoclonal Antibody (Product # MA5-38652) and DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of TWIST1 in paraffin-embedded colon cancer tissues using TWIST1 Monoclonal Antibody (Product # MA5-38652) and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis of TWIST1 in HeLa cells using TWIST1 Monoclonal Antibody (Product # MA5-38652) (green) and negative control (red).