Antibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [3]
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- Product number
- ALX-804-149-C100 - Provider product page
- Provider
- Enzo Life Sciences
- Proper citation
- Enzo Life Sciences Cat#ALX-804-149-C100, RRID:AB_2050733
- Product name
- APRIL (human) monoclonal antibody (Aprily-8)
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein fragment
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- Aprily-8
- Vial size
- 100 μg
- Storage
- -20°C
- Handling
- Avoid freeze/thaw cycles.
Submitted references Expression of TNF-superfamily members BAFF and APRIL in breast cancer: immunohistochemical study in 52 invasive ductal breast carcinomas.
Pelekanou V, Kampa M, Kafousi M, Darivianaki K, Sanidas E, Tsiftsis DD, Stathopoulos EN, Tsapis A, Castanas E
BMC cancer 2008 Mar 20;8:76
BMC cancer 2008 Mar 20;8:76
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Supportive validation
- Submitted by
- Enzo Life Sciences (provider)
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- Experimental details
- Western blot of total cell extracts from HEK 293 cells transfected with the indicated expression vector. Aprily-8 reacts specifically with human APRIL. Method: 10µg of protein was applied to the gel. Revealed with Aprily-8 (1µg/ml) and HRP-coupled anti-mouse secondary antibody (1:4'000).Note: The human APRIL construct used in this experiment is an uncleavable fusion protein between human BAFF (aa 1-132) and human APRIL (aa 93-233).
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- FACS analysis of cells with MAb to APRIL (Aprily-8). Method: HEK 293 cells were mock transfected or transfected with an expression plasmid coding for a non-cleavable human APRIL. Cells (5 x 10E5) were incubated on ice for 30 min. in 50µl FACS buffer (PBS, 5% fetal calf serum, 0.02% azide) containing 10µg/ml of Aprily-8 antibody. After washing in FACS buffer, FITC-conjugated antibody to mouse IgG was added. Cells were incubated on ice for 30 min., washed and analyzed by flow cytometry.
- Submitted by
- Enzo Life Sciences (provider)
- Main image
- Experimental details
- Immunostaining of HEK 293 cells transfected with a human APRIL expression plasmid (left panels), or mock transfected (right panel) by Aprily-8. Method: 3 days after transfection of cells with the indicated constructs, cells were fixed with 4% formaldehyde 5 min. at RT. After a wash in PBS, samples were dehydrated by washes in 60%, 80%, 90%, 100% EtOH and xylol. Cells were then dried and embedded in paraffin. Sections were cut, mounted on slides and dried overnight at 50¡C. Slides were then successively washed 2x 10 min. in xylol, 2x 10 min. in 100% ethanol, and then treated 10 min. in 100% methanol/0.6% H202 to inhibit endogenous peroxidase. Samples were rehydrated by washes in 90%, 80%, 60% ethanolÊand PBS. After micro-wave treatment, slides were washed 3x in PBS, blocked with IgG, and incubated for 1 hour with 5µg/ml Aprily-8 or control mouse IgG (isotype control) in 1%BSA / 1x PBS for 1 hour. After PBS washes, samples were incubated with the secondary Ab for 1 hour, washed in PBS and revealed with StreptABComplex/HRP (Vector) and AEC.