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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 627203 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#627203, RRID:AB_2565761
- Product name
- PE anti-TRIM
- Antibody type
- Monoclonal
- Antigen
- Partial recombinant human TRIM (amino acids 29-186)
- Description
- TRIM is a 30 kD disulfide-linked protein also known as T cell receptor interacting molecule. TRIM is a type III membrane protein containing a very short extracellular domain (8 amino acids). TRIM is highly expressed in T cells with some expression also observed in NK cells. TRIM is a signaling adaptor molecule that links extracellular and intracellular signaling. The TRIM protein is phosphorylated by Src kinases and associates with the PI3K regulatory subunit α after phosphorylation. TRIM has also been shown to associate with TCRα, β, and ζ. The TRIM-4 monoclonal antibody recognizes human TRIM and has been shown to be useful for Western blotting, intracellular staining by flow cytometry, and immunofluorescence staining.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- TRIM-4
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Human peripheral blood lymphocytes were treated with True-Nuclear™ Transcription Factor Buffer Set. Cells were then stained with TRIM (clone TRIM-4) PE (filled histogram) or mouse IgG2a, κ PE isotype control (open histogram).
- Conjugate
- Yellow dye
