MA5-44779

antibody from Invitrogen Antibodies

Targeting: TRIP10 CIP4, HSTP, STOT, STP

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-44779

Provider

Invitrogen Antibodies

Product name

TRIP10 Recombinant Rabbit Monoclonal Antibody (JE63-45)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

JE63-45

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cip4 in different lysates. Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in Cip4 Monoclonal antibody (Product # MA5-44779) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Lane 1: JAR cell lysate (10 µg/Lane); Lane 2: HepG2 cell lysate (10 µg/Lane); Lane 3: 293T cell lysate (10 µg/Lane); Lane 4: NIH/3T3 cell lysate (10 µg/Lane); Lane 5: Mouse colon tissue lysate (20 µg/Lane). Predicted band size: 68 kDa. Observed band size: 80 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Cip4 in different lysates. Exposure time: 2 minutes; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. Samples were incubated in Cip4 Monoclonal antibody (Product # MA5-44779) using a dilution of 1:500 in 5% NFDM/TBST at room temperature for 2 hours followed by Goat Anti-Rabbit IgG - HRP secondary antibody at a dilution of 1:200,000 for 1 hour at room temperature. Lane 1: PC-12 cell lysate (10 µg/Lane); Lane 2: Rat brain tissue lysate (20 µg/Lane). Predicted band size: 68 kDa. Observed band size: 74 kDa.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cip4 in paraffin-embedded human testis tissue. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with Cip4 Monoclonal antibody (Product # MA5-44779) using a dilution of 1:100 for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.