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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunoprecipitation [1]
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- Product number
- NB100-59833 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-59833, RRID:AB_2209247
- Product name
- Rabbit Polyclonal Cip4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Cip4 Antibody [NB100-59833] - Detection of Human TRIP10 on HeLa whole cell lysate using NB100-59833. TRIP10 was also immunoprecipitated by rabbit anti-TRIP10 antibody NB100-59834.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Cip4 Antibody [NB100-59833] - Detection of human TRIP10/CIP4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-TRIP10/CIP4 antibody NB100-59833 (lot NB100-59833-2) used for IP at 6 ug per reaction. TRIP10/CIP4 was also immunoprecipitated by a previous lot of this antibody (NB100-59833-1) and rabbit anti-TRIP10/CIP4 antibody NB100-59834. Detection: Chemiluminescence with an exposure time of 3 minutes.
