H00065110-M06

antibody from Abnova Corporation

Targeting: UPF3A HUPF3A, RENT3A, UPF3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00065110-M06

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00065110-M06, RRID:AB_1237902

Product name

UPF3A monoclonal antibody (M06), clone 2E2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant UPF3A.

Antigen sequence

TGGGKQESCAPGAVVKARPMEGSLEEPQETSHSGS
DKEHRDVERSQEQESEAQRYHVDDGRRHRAHHEPE
RLSRRSEDEQRWGKGPGQDRGKKGSQDSGAP


Isotype

IgG

Antibody clone number

2E2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of UPF3A expression in transfected 293T cell line by UPF3A monoclonal antibody (M06), clone 2E2.Lane 1: UPF3A transfected lysate(51 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged UPF3A is 0.03 ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal antibody to UPF3A on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml]

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Protocol

Protocol