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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- PAB9925 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9925, RRID:AB_1672065
- Product name
- ABCB5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of ABCB5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Association between tumorigenic potential and the fate of cancer cells in a syngeneic melanoma model.
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma.
Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.
Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes.
Krelin Y, Berkovich L, Amit M, Gil Z
PloS one 2013;8(4):e62124
PloS one 2013;8(4):e62124
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma.
Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH
Cancer research 2005 May 15;65(10):4320-33
Cancer research 2005 May 15;65(10):4320-33
Useful tool to generate unidirectional deletion vectors by utilizing the star activity of BamHI in an NcoI-BamHI-XhoI cassette.
Chen KG, Gottesman MM
BioTechniques 2005 Feb;38(2):198, 200, 202, 204
BioTechniques 2005 Feb;38(2):198, 200, 202, 204
Principal expression of two mRNA isoforms (ABCB 5alpha and ABCB 5beta ) of the ATP-binding cassette transporter gene ABCB 5 in melanoma cells and melanocytes.
Chen KG, Szakács G, Annereau JP, Rouzaud F, Liang XJ, Valencia JC, Nagineni CN, Hooks JJ, Hearing VJ, Gottesman MM
Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society 2005 Apr;18(2):102-12
Pigment cell research / sponsored by the European Society for Pigment Cell Research and the International Pigment Cell Society 2005 Apr;18(2):102-12
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using ABCB5 polyclonal antibody (Cat # PAB9925) shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate.No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown).A 3-8% Tris-acetate gel was used for separation.The arrowhead corresponds to 117 kDa ABCB5.The membrane was probed with the primary antibody at a 1 : 10,000 dilution in 5% milk in TBST at 4°C, overnight.Personal Communication, JP Gillet, CCR-NCI, Bethesda, MD.
