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Western blot
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- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
- Flow cytometry [1]
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- Product number
- NB100-93365 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-93365, RRID:AB_1236601
- Product name
- Goat Polyclonal ABCB5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise both reported isoforms (NP_848654.3
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
C-QTQHRNTSKKAQ- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Granulin-epithelin precursor and ATP-dependent binding cassette (ABC)B5 regulate liver cancer cell chemoresistance.
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma.
Cheung ST, Cheung PF, Cheng CK, Wong NC, Fan ST
Gastroenterology 2011 Jan;140(1):344-55
Gastroenterology 2011 Jan;140(1):344-55
ABCB5-mediated doxorubicin transport and chemoresistance in human malignant melanoma.
Frank NY, Margaryan A, Huang Y, Schatton T, Waaga-Gasser AM, Gasser M, Sayegh MH, Sadee W, Frank MH
Cancer research 2005 May 15;65(10):4320-33
Cancer research 2005 May 15;65(10):4320-33
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ABCB5 Antibody [NB100-93365] - (0.3ug/ml) staining of lysates of cell line A431 (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ABCB5 Antibody [NB100-93365] - analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
