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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- NB300-987 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-987, RRID:AB_2197651
- Product name
- Goat Polyclonal STAP2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognise isoforms 1 (NP_060190.2) and isoform 2 (NP_001013863.1).
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Involvement of STAP-2 in Brk-mediated phosphorylation and activation of STAT5 in breast cancer cells.
Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells.
STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation.
Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells.
STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif.
Ikeda O, Mizushima A, Sekine Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
Cancer science 2011 Apr;102(4):756-61
Cancer science 2011 Apr;102(4):756-61
Interactions of STAP-2 with Brk and STAT3 participate in cell growth of human breast cancer cells.
Ikeda O, Sekine Y, Mizushima A, Nakasuji M, Miyasaka Y, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
The Journal of biological chemistry 2010 Dec 3;285(49):38093-103
The Journal of biological chemistry 2010 Dec 3;285(49):38093-103
STAP-2 is phosphorylated at tyrosine-250 by Brk and modulates Brk-mediated STAT3 activation.
Ikeda O, Miyasaka Y, Sekine Y, Mizushima A, Muromoto R, Nanbo A, Yoshimura A, Matsuda T
Biochemical and biophysical research communications 2009 Jun 19;384(1):71-5
Biochemical and biophysical research communications 2009 Jun 19;384(1):71-5
Signal-transducing adaptor protein-2 regulates stromal cell-derived factor-1 alpha-induced chemotaxis in T cells.
Sekine Y, Ikeda O, Tsuji S, Yamamoto C, Muromoto R, Nanbo A, Oritani K, Yoshimura A, Matsuda T
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7966-74
Journal of immunology (Baltimore, Md. : 1950) 2009 Dec 15;183(12):7966-74
STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif.
Minoguchi M, Minoguchi S, Aki D, Joo A, Yamamoto T, Yumioka T, Matsuda T, Yoshimura A
The Journal of biological chemistry 2003 Mar 28;278(13):11182-9
The Journal of biological chemistry 2003 Mar 28;278(13):11182-9
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAP2 Antibody [NB300-987] - (0.03ug/ml) staining of human heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: STAP2 Antibody [NB300-987] - NB300-987 (0.03ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: STAP2 Antibody [NB300-987] - (3.8ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
