Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- BS3425 - Provider product page
- Provider
- Bioworld Technology, Inc
- Proper citation
- Bioworld Technology Cat#BS3425, RRID:AB_1662138
- Product name
- EMR1 (T860) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide, corresponding to amino acids 826-880 of Human EMR1.
- Description
- The epidermal growth factor (EGF)-TM7 family constitutes a group of class B G protein-coupled receptors, which includes CD97, EMR1(EGF-like molecule containing mucin-like hormone receptor 1, designated F4/80 in mouse), EMR2, EMR3, FIRE and ETL. These family members are characterized by an extended extracellular region with several N-terminal EGF domains, and are predominantly expressed on cells of the immune system. The EGF-TM7 protein family are encoded by a gene cluster on human chromosome 19p13. The F4/80 molecule is solely expressed on the surface of macrophages and serves as a marker for mature macrophage tissues, including Kupffer cells in liver, splenic red pulp macrophages, brain microglia, gut lamina propria and Langerhans cells in the skin. It is detected as 160 kDa protein that undergoes extensive N-linked glycosylation as well as some O-linked glycosylation. The function of F4/80/EMR1 is unclear, but it is speculated to be involved in macrophage adhesion events, cell migration or as a G protein-coupled signaling component of macrophages.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100ul
- Concentration
- 1 mg/ml
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Bioworld Technology, Inc (provider)
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- Experimental details
- Western blot (WB) analysis of EMR1 (T860) pAb at 1:500 dilutionLane1:A549 whole cell lysateLane2:sp2/0 whole cell lysateLane3:PC12 whole cell lysate