- PA5-32399 - Invitrogen Antibodies ADGRE1 antibody

Submitted references siRNA against CD40 delivered via a fungal recognition receptor ameliorates murine acute graft-versus-host disease.

Heissig B, Salama Y, Tateno M, Takahashi S, Hattori K
EJHaem 2022 Aug;3(3):849-861

The Transcription Factor SUB1 Is a Master Regulator of the Macrophage TLR Response in Atherosclerosis.

Huang R, Hu Z, Chen X, Cao Y, Li H, Zhang H, Li Y, Liang L, Feng Y, Wang Y, Su W, Kong Z, Melgiri ND, Jiang L, Li X, Du J, Chen Y
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2021 Oct;8(19):e2004162

The influence of M-CSF on fracture healing in a mouse model.

Starlinger J, Sarahrudi K, Kecht M, Koerbler F, Pietschmann P, Aharinejad S
Scientific reports 2021 Nov 16;11(1):22326

The role of inhibition of osteocyte apoptosis in mediating orthodontic tooth movement and periodontal remodeling: a pilot study.

Kaplan M, Kalajzic Z, Choi T, Maleeh I, Ricupero CL, Skelton MN, Daily ML, Chen J, Wadhwa S
Progress in orthodontics 2021 Jul 26;22(1):21

Regulation of adipose tissue inflammation and systemic metabolism in murine obesity by polymer implants loaded with lentiviral vectors encoding human interleukin-4.

Youngblood R, Flesher CG, Delproposto J, Baker NA, Neeley CK, Li F, Lumeng CN, Shea LD, O'Rourke RW
Biotechnology and bioengineering 2020 Dec;117(12):3891-3901

Mitochondrial calcium uniporter regulates PGC-1α expression to mediate metabolic reprogramming in pulmonary fibrosis.

Gu L, Larson Casey JL, Andrabi SA, Lee JH, Meza-Perez S, Randall TD, Carter AB
Redox biology 2019 Sep;26:101307

Sub-clinical dose of bone morphogenetic protein-2 does not precipitate rampant, sustained inflammatory response in bone wound healing.

Grey ZJ, Howie RN, Durham EL, Hall SR, Helke KL, Steed MB, LaRue AC, Muise-Helmericks RC, Cray JJ
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society 2019 Jul;27(4):335-344

Genomic binding of PAX8-PPARG fusion protein regulates cancer-related pathways and alters the immune landscape of thyroid cancer.

Zhang Y, Yu J, Grachtchouk V, Qin T, Lumeng CN, Sartor MA, Koenig RJ
Oncotarget 2017 Jan 24;8(4):5761-5773

High-Fat Diet Promotion of Endometriosis in an Immunocompetent Mouse Model is Associated With Altered Peripheral and Ectopic Lesion Redox and Inflammatory Status.

Heard ME, Melnyk SB, Simmen FA, Yang Y, Pabona JM, Simmen RC
Endocrinology 2016 Jul;157(7):2870-82

Krüppel-like factor 9 (KLF9) prevents colorectal cancer through inhibition of interferon-related signaling.

Brown AR, Simmen RC, Raj VR, Van TT, MacLeod SL, Simmen FA
Carcinogenesis 2015 Sep;36(9):946-55

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemical analysis of F4/80 using anti-F4/80 Polyclonal Antibody (Product # PA5-32399) in Human Lung Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:100.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 6 Immunostaining on macrophage marker F4/80 + on the compression side. There appeared to be an increased number of macrophages (stained brown) in orthodontically loaded group treated with saline ( A ) compared to orthodontically loaded group treated with IG9402 (stained less intensively) ( B ) (arrows). C Negative control, primary antibody omitted

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 5 Immunohistochemical staining for F4/80 and CD3e in thyroid glands from PPFPThy;PtenThy-/- and PtenThy-/- mice fed pioglitazone or control diet

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: 1 Figure Transplantation of adipose tissue with hIL-4 PLG implants. (a) Transplantation of implants into eWAT fat pads; left-implants placed in each eWAT fat pad before folding fat pad over the implant and returning to abdominal cavity: right--implants in eWAT fat pads 2 weeks after transplantation. (b) Study design timeline. (c) Expression of hIL-4 in murine adipose tissue measured by qRT-PCR at 2 and 10 weeks after implantation; ordinate: fold difference in transcript level relative to sham mice; * p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 Immunostaining on macrophage marker F4/80 + on the compression side. There appeared to be an increased number of macrophages (stained brown) in orthodontically loaded group treated with saline ( A ) compared to orthodontically loaded group treated with IG9402 (stained less intensively) ( B ) (arrows). C Negative control, primary antibody omitted

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 6 Transplantation of Sub1 knockout macrophages reduces Western diet-induced atherosclerosis in Ldlr -/- recipient mice. Irradiated Ldlr -/- mice transplanted with bone marrow from Sub1 flox/flox mice (WT- Ldlr -/- ), LysM Cre/- / Sub1 flox/wt mice (HEMI- Ldlr -/- ), LysM Cre/- / Sub1 flox/flox mice ( Sub1 KO- Ldlr -/- ), or LysM Cre/- / Sub1 flox/flox ; Stat6 -/- mice ( Sub1 , Stat6 KO- Ldlr -/- ) were fed a Western diet for 12 weeks. A) Representative H&E and Oil Red O staining images showing lesion areas in aortic root sections. Scale bar = 200 um. Quantification of aortic root lesion areas based on 8-12 10 um sections per mouse (30 um apart). B) Quantification of total lesion areas in en face aortas. C) Representative Masson's trichrome staining images and quantification of aortic root lesion collagen by Zeiss Axiovision software. Scale bar = 200 um. D) qPCR of M1 marker genes in isolated aortic root plaque macrophages. E) In vitro bone marrow-derived macrophages (BMDMs) proliferation under vehicle (Ctrl) or oxLDL (50 ug mL -1 , 24 h) conditions and F) in vivo F4/80+ macrophage proliferation in aortic root lesions examined using anti-Ki67 immunofluorescence. Scale bar = 100 um. G) In vitro BMDM apoptosis levels under vehicle (Ctrl) or oxLDL (50 ug mL -1 , 24 h) conditions assessed by TUNEL staining. Cell morphology analyzed by differential interference contrast (DIC) and nuclear staining by DAPI. Apoptotic cell percentage expressed as ratio of TUNEL+/DAPI+. Scale ba

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 F4/80 staining ( A - C ) visualizing monocytes and TRAP staining ( D - F ) visualizing osteoclasts of exemplary slices of the three groups of wildtype mice are show here. Corresponding uCT pictures are shown in ( G - I ). ( A ) 6D and 6G correspond to mice treated with OT (ot) and external fixator (group 1). ( B , E , H ) represent mice treated with OT (osteotomy), external fixator and M-CSF (group 2). ( C , F , I ) represent mice treated with OT only (group 3). To visualize osteoclasts, the sections were stained for tartrate resistant acid phosphatase (TRAP, Sigma) and counterstained with toluidine blue. Monocytes were visualized using F4/80 antibodies (Invitrogen PA5-32399), then treated with HRP (Dako EnVision system, Dako A/S, Glostrup, Denmark) and ultimately counterstained with hemalum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: FIGURE 4 Targeting CD40 using the small interfering RNA (siRNA) compounds did not prevent skin acute graft-versus-host disease (aGvHD). (A) Hematoxylin and eosin (H&E)-stained liver and spleen tissue sections of different treatment groups at indicated time points. Magnification of main image (bar = 100 mum). (B) Representative images of immunoreactive CD11b cells in liver tissue sections of allogenic bone marrow transplantation (BMT) mice treated with carrier or NJA-312 (magnification bar = 100 mum). (C) Representative images of skin sections from animals 34 days after BMT stained with CD11b (blue signal) and co-stained with F4/80 (brown signal) (bar = 100 mum). Arrows indicate examples of positive cells in both sections. (D) Representative images of skin sections from animals 34 days after BMT stained with CD3 (red signal) in the upper panels (bar = 100 mum). Arrows indicate examples of CD3+ cells in all images. Isotype controls are given

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 MCU expression and mitochondrial Ca 2+ are increased in IPF lung macrophages. (A) Total RNA was isolated from lung macrophages of normal or IPF subjects. MCU mRNA was measured by real-time PCR, n = 10. (B) MCU was detected by immunoblot analysis in lung macrophage mitochondria from human subjects. (C) Statistical analysis, n = 6. (D) Ca 2+ was measured in isolated mitochondria of lung macrophages from human subjects, n = 8. (E) The colocalization of MCU and F4/80 in lung tissue of normal and IPF subjects was detected by IHC-P. Representative micrograph of three individual subjects. Scale bars, 20 mum. (F) The colocalization of MCU and SPC in lung tissue from normal and IPF subjects was detected by IHC-P. Representative micrograph of three individual subjects. Scale bars, 20 mum. (G) WT mice were exposed to saline or bleomycin (1.75U/kg). Lung macrophages were harvested by BAL at indicated time points (day 5, 10, 15 and 21). Whole cell lysate was prepared for the detection of MCU by immuoblot analysis. (H) Reciprocal bone marrow (BM) chimera was performed with WT and MCU +- mice. After 8 weeks mice were exposed to saline or bleomycin (1.75U/kg) for 21 days. Lungs were subjected to Sirius red staining. Represented micrographs from 6 mice per condition are shown. Scale bars, 200 mum at x5. (I) Lung collagen content was determined by hydroxyproline assay, n = 6. WT and DN-MCU-Lyz2-cre littermates were exposed to saline or bleomycin for 21 days. (J) Mitochondrial Ca 2+ in l

PA5-32399

Antibody data