Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- PAB1263 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1263, RRID:AB_1204609
- Product name
- Lat2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant Lat2.
- Storage
- Store at 4°C. Do not freeze.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blotting analysis (non - reducing conditions) of mouse Lat2 in mouse spleen tissue. Lane 1 : Immunostaining with Lat2 polyclonal antibody (Cat # PAB1263) . Lane 2 : Immunostaining with Lat2 polyclonal antibody (specific for N - terminal domain of mouse Lat2) .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Distribution of LAT and Lat2 in separate clusters on mast cell plasma membrane sheets. LAT and Lat2 were detected on mouse bone marrow - derived mast cells (BMMC ; A) and RBL rat basophilic leukemia cell line (B) by anti - LAT (LAT - 1D1) and Lat2 polyclonal antibody, followed by 5 or 10 nm gold - conjugated secondary antibodies, respectively.
- Validation comment
- Immunocytochemistry