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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [2]
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- Product number
- PA5-47518 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- HES4 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, this antibody shows less than 1% cross-reactivity with recombinant human (rh) HES-1, rhHES-2, rhHES-5, and rhHES-7. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HES4 in immersion fixed HEK293 human embryonic kidney cell line. Samples were incubated in HES4 polyclonal antibody (Product # PA5-47518) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of HES4 in immersion fixed HEK293 human embryonic kidney cell line. Samples were incubated in HES4 polyclonal antibody (Product # PA5-47518) using a dilution of 1.7 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.
