HPA023505

antibody from Atlas Antibodies

Targeting: ATF7IP FLJ10688, p621

Western blot (Enhanced validation)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA023505

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA023505, RRID:AB_1845148

Product name

Anti-ATF7IP

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

SMEIDQGEKNEDETSADLVETINENVIEDNKSENI
LENTDSMETDEIIPILEKLAPSEDELTCFSKTSLL
PIDETNPDLEEKMESSFGSPSKQESSESLPKEAFL
VLSDEEDISGEKDESEVISQNETCSP


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Western blot

Enhanced validation

Submitted by

klas2

Enhanced method

Genetic validation

Main image

Experimental details

Western blot of cell lysate from U-2 OS cells transfected with either siRNA targeting ATF7IP or control siRNA. Lane 1: Marker (250, 130, 95, 72, 55, 36, 28, 17, 10) Lane 2: Cell lysate from U-2OS cells transfected with siRNA targeting ATF7IP Lane 3: N/A Lane 4: Cell lysate from U-2OS cells transfected with control siRNA Right image, lane 1-4: loading control

Sample type

U-2 OS

Primary Ab dilution

1:383

Conjugate

Horseradish Peroxidase

Secondary Ab

Secondary Ab

Secondary Ab dilution

1:3000

Knockdown/Genetic Approaches Application

Western blot

KD Reagent Type

siRNA

Downregulation

>75%

KD Reagent Prod page

KD Reagent Prod page

KD Reagent Prod no

s31356

KD Reagent Provider

Thermo Fisher Scientific

KD Reagent Prod no 2

s31357

KD Reagent Page 2

KD Reagent Page 2

KD Reagent Prod no 3

s31358

KD Reagent Page 3

KD Reagent Page 3

Antibody Lot Number

R09412

Appendix

566bfc3f0d05f.pdf

Show more

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATF7IP antibody. Remaining relative intensity is presented.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis in A-431 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ATF7IP antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nuclear bodies.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA023505 antibody. Corresponding ATF7IP RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human esophagus shows strong nuclear positivity in squamous epithelial cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low expression as expected.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows moderate nuclear positivity in glandular cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human lymph node shows moderate nuclear positivity in germinal center cells.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skeletal muscle shows low nuclear positivity in myocytes as expected.

Sample type

HUMAN