Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-438 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-438, RRID:AB_2059058
- Product name
- Rabbit Polyclonal MCAF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references In vivo epigenetic editing of Sema6a promoter reverses transcallosal dysconnectivity caused by C11orf46/Arl14ep risk gene.
Peter CJ, Saito A, Hasegawa Y, Tanaka Y, Nagpal M, Perez G, Alway E, Espeso-Gil S, Fayyad T, Ratner C, Dincer A, Gupta A, Devi L, Pappas JG, Lalonde FM, Butman JA, Han JC, Akbarian S, Kamiya A
Nature communications 2019 Sep 11;10(1):4112
Nature communications 2019 Sep 11;10(1):4112
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAF1 Antibody [NB100-438] - Nuclear extract (20 ug) from HeLa cells. Antibody used at 0.2 and 0.1 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCAF1 Antibody [NB100-438] - Detection of Human MCAF by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MCAF antibody NB100-438 used for WB at 0.05 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MCAF1 Antibody [NB100-438] - FFPE section of human small cell lung cancer. NB100-438 used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: MCAF1 Antibody [NB100-438] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MCAF used at a dilution of 1:1,000 (1ug/ml). Detection: DAB