- GTX110527 - GeneTex IL6 antibody

Submitted references M2 macrophage-derived IL6 mediates resistance of breast cancer cells to hedgehog inhibition.

Xu X, Ye J, Huang C, Yan Y, Li J
Toxicology and applied pharmacology 2019 Feb 1;364:77-82

In vitro studies on anti-inflammatory activities of kiwifruit peel extract in human THP-1 monocytes.

D'Eliseo D, Pannucci E, Bernini R, Campo M, Romani A, Santi L, Velotti F
Journal of ethnopharmacology 2019 Apr 6;233:41-46

17β-estradiol upregulates IL6 expression through the ERβ pathway to promote lung adenocarcinoma progression.

Huang Q, Zhang Z, Liao Y, Liu C, Fan S, Wei X, Ai B, Xiong J
Journal of experimental & clinical cancer research : CR 2018 Jul 3;37(1):133

Vascular niche IL-6 induces alternative macrophage activation in glioblastoma through HIF-2α.

Wang Q, He Z, Huang M, Liu T, Wang Y, Xu H, Duan H, Ma P, Zhang L, Zamvil SS, Hidalgo J, Zhang Z, O'Rourke DM, Dahmane N, Brem S, Mou Y, Gong Y, Fan Y
Nature communications 2018 Feb 8;9(1):559

Cytokine expression associated with Helicobacter pylori and Epstein-Barr virus infection in gastric carcinogenesis.

Kim JY, Bae BN, Kang G, Kim HJ, Park K
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2017 Sep;125(9):808-815

Fisetin Regulates Nrf2 Expression and the Inflammation-Related Signaling Pathway to Prevent UVB-Induced Skin Damage in Hairless Mice.

Wu PY, Lyu JL, Liu YJ, Chien TY, Hsu HC, Wen KC, Chiang HM
International journal of molecular sciences 2017 Oct 10;18(10)

A standardized herbal extract mitigates tumor inflammation and augments chemotherapy effect of docetaxel in prostate cancer.

Tsai CH, Tzeng SF, Hsieh SC, Yang YC, Hsiao YW, Tsai MH, Hsiao PW
Scientific reports 2017 Nov 15;7(1):15624

Therapeutic effect of urine-derived stem cells for protamine/lipopolysaccharide-induced interstitial cystitis in a rat model.

Li J, Luo H, Dong X, Liu Q, Wu C, Zhang T, Hu X, Zhang Y, Song B, Li L
Stem cell research & therapy 2017 May 8;8(1):107

IL-5 and IL-6 are increased in the frontal recess of eosinophilic chronic rhinosinusitis patients.

Kubota K, Takeno S, Taruya T, Sasaki A, Ishino T, Hirakawa K
Journal of otolaryngology - head & neck surgery = Le Journal d'oto-rhino-laryngologie et de chirurgie cervico-faciale 2017 May 2;46(1):36

Role of Expression of Inflammatory Mediators in Primary and Recurrent Lumbar Disc Herniation.

Dagistan Y, Cukur S, Dagistan E, Gezici AR
Journal of Korean Neurosurgical Society 2017 Jan 1;60(1):40-46

Secretory RAB GTPase 3C modulates IL6-STAT3 pathway to promote colon cancer metastasis and is associated with poor prognosis.

Chang YC, Su CY, Chen MH, Chen WS, Chen CL, Hsiao M
Molecular cancer 2017 Aug 7;16(1):135

Cyclophosphamide-induced HCN1 channel upregulation in interstitial Cajal-like cells leads to bladder hyperactivity in mice.

Liu Q, Long Z, Dong X, Zhang T, Zhao J, Sun B, Zhu J, Li J, Wang Q, Yang Z, Hu X, Li L
Experimental & molecular medicine 2017 Apr 21;49(4):e319

Chitosan Treatment Delays the Induction of Senescence in Human Foreskin Fibroblast Strains.

Tsai CW, Kao YT, Chiang IN, Wang JH, Young TH
PloS one 2015;10(10):e0140747

Anti-inflammatory Effects of Resveratrol on Hypoxia/Reoxygenation-Induced Alveolar Epithelial Cell Dysfunction.

Liu PL, Chong IW, Lee YC, Tsai JR, Wang HM, Hsieh CC, Kuo HF, Liu WL, Chen YH, Chen HL
Journal of agricultural and food chemistry 2015 Nov 4;63(43):9480-7

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Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A549 B: HeLa C: HepG2 D: HCT116 12% SDS PAGE GTX110527 diluted at 1:1000

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:1500 and competitor's antibody (CST#12153) diluted by 1:3000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: IL6 antibody detects IL6 recombinant protein by western blot analysis. Various amounts of protein were separated by 12% SDS-PAGE, and the membranes were blotted with IL6 antibody (GTX110527) diluted at 1:2250 and competitor's antibody (CST#12153) diluted by 1:2000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX110527) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of methanol-fixed HeLa, using IL6(GTX110527) antibody at 1:200 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded human breast cancer, using IL6(GTX110527) antibody at 1:250 dilution.

GTX110527

Antibody data