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- Antibody Data
- Antigen structure
- References [55]
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- Western blot [1]
- Immunohistochemistry [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB206-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Primate IL-6 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human and primate IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL-6, rhOSM, rhLIF, rhIL-11, rhgp130, or rhCNTF.
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 6708
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Early Loss of Histone H2B Monoubiquitylation Alters Chromatin Accessibility and Activates Key Immune Pathways That Facilitate Progression of Ovarian Cancer.
The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies.
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis.
Age-Related Changes to Human Tear Composition.
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma.
Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets.
Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line.
Bone Metastasis of Prostate Cancer Can Be Therapeutically Targeted at the TBX2-WNT Signaling Axis.
Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration.
Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway.
IL-6 trans-signaling is another pathway to upregulate Osteopontin.
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib.
Monocyte Subsets Coregulate Inflammatory Responses by Integrated Signaling through TNF and IL-6 at the Endothelial Cell Interface.
Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium.
CXCL8 and CCL20 Enhance Osteoclastogenesis via Modulation of Cytokine Production by Human Primary Osteoblasts.
Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Epstein-Barr virus infection induces indoleamine 2,3-dioxygenase expression in human monocyte-derived macrophages through p38/mitogen-activated protein kinase and NF-κB pathways: impairment in T cell functions.
Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
Digital microfluidic assay for protein detection.
Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner.
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
Protection from RNA and DNA viruses by IL-32.
Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome.
Endogenous IL-32 controls cytokine and HIV-1 production.
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
Ultrasensitive flow-based immunoassays using single-molecule counting.
Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
trans fatty acids and systemic inflammation in heart failure.
Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation.
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
Hooda J, Novak M, Salomon MP, Matsuba C, Ramos RI, MacDuffie E, Song M, Hirsch MS, Lester J, Parkash V, Karlan BY, Oren M, Hoon DS, Drapkin R
Cancer research 2019 Feb 15;79(4):760-772
Cancer research 2019 Feb 15;79(4):760-772
The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by α-PD-L1 or α-IL6 antibodies.
Rolvering C, Zimmer AD, Ginolhac A, Margue C, Kirchmeyer M, Servais F, Hermanns HM, Hergovits S, Nazarov PV, Nicot N, Kreis S, Haan S, Behrmann I, Haan C
Journal of leukocyte biology 2018 Nov;104(5):969-985
Journal of leukocyte biology 2018 Nov;104(5):969-985
Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases.
Gril B, Paranjape AN, Woditschka S, Hua E, Dolan EL, Hanson J, Wu X, Kloc W, Izycka-Swieszewska E, Duchnowska R, Pęksa R, Biernat W, Jassem J, Nayyar N, Brastianos PK, Hall OM, Peer CJ, Figg WD, Pauly GT, Robinson C, Difilippantonio S, Bialecki E, Metellus P, Schneider JP, Steeg PS
Nature communications 2018 Jul 13;9(1):2705
Nature communications 2018 Jul 13;9(1):2705
Exposure to wild-type AAV drives distinct capsid immunity profiles in humans.
Kuranda K, Jean-Alphonse P, Leborgne C, Hardet R, Collaud F, Marmier S, Costa Verdera H, Ronzitti G, Veron P, Mingozzi F
The Journal of clinical investigation 2018 Dec 3;128(12):5267-5279
The Journal of clinical investigation 2018 Dec 3;128(12):5267-5279
Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy.
Logue SE, McGrath EP, Cleary P, Greene S, Mnich K, Almanza A, Chevet E, Dwyer RM, Oommen A, Legembre P, Godey F, Madden EC, Leuzzi B, Obacz J, Zeng Q, Patterson JB, Jäger R, Gorman AM, Samali A
Nature communications 2018 Aug 15;9(1):3267
Nature communications 2018 Aug 15;9(1):3267
Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis.
Touil H, Kobert A, Lebeurrier N, Rieger A, Saikali P, Lambert C, Fawaz L, Moore CS, Prat A, Gommerman J, Antel JP, Itoyama Y, Nakashima I, Bar-Or A, Canadian B Cell Team in MS.
Journal of neuroinflammation 2018 Apr 19;15(1):114
Journal of neuroinflammation 2018 Apr 19;15(1):114
Age-Related Changes to Human Tear Composition.
Micera A, Di Zazzo A, Esposito G, Longo R, Foulsham W, Sacco R, Sgrulletta R, Bonini S
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2024-2031
Investigative ophthalmology & visual science 2018 Apr 1;59(5):2024-2031
IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3.
Lv R, Zhao J, Lei M, Xiao D, Yu Y, Xie J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology 2017;42(5):1961-1972
Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma.
Brencicova E, Jagger AL, Evans HG, Georgouli M, Laios A, Attard Montalto S, Mehra G, Spencer J, Ahmed AA, Raju-Kankipati S, Taams LS, Diebold SS
PloS one 2017;12(4):e0175712
PloS one 2017;12(4):e0175712
Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets.
Galland S, Vuille J, Martin P, Letovanec I, Caignard A, Fregni G, Stamenkovic I
Cell reports 2017 Sep 19;20(12):2891-2905
Cell reports 2017 Sep 19;20(12):2891-2905
Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line.
Ortiz-Montero P, Londoño-Vallejo A, Vernot JP
Cell communication and signaling : CCS 2017 May 4;15(1):17
Cell communication and signaling : CCS 2017 May 4;15(1):17
Bone Metastasis of Prostate Cancer Can Be Therapeutically Targeted at the TBX2-WNT Signaling Axis.
Nandana S, Tripathi M, Duan P, Chu CY, Mishra R, Liu C, Jin R, Yamashita H, Zayzafoon M, Bhowmick NA, Zhau HE, Matusik RJ, Chung LW
Cancer research 2017 Mar 15;77(6):1331-1344
Cancer research 2017 Mar 15;77(6):1331-1344
Interleukin‑6 induces an epithelial‑mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration.
Zhou J, Zhang C, Pan J, Chen L, Qi ST
Molecular medicine reports 2017 Jun;15(6):4123-4131
Molecular medicine reports 2017 Jun;15(6):4123-4131
Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway.
Wang D, Tang S, Zhang Q
Oncology letters 2017 Jun;13(6):4875-4881
Oncology letters 2017 Jun;13(6):4875-4881
IL-6 trans-signaling is another pathway to upregulate Osteopontin.
Uchibori T, Matsuda K, Shimodaira T, Sugano M, Uehara T, Honda T
Cytokine 2017 Feb;90:88-95
Cytokine 2017 Feb;90:88-95
Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory "FADDosome" Complex upon TRAIL Stimulation.
Henry CM, Martin SJ
Molecular cell 2017 Feb 16;65(4):715-729.e5
Molecular cell 2017 Feb 16;65(4):715-729.e5
Sensitization of EGFR Wild-Type Non-Small Cell Lung Cancer Cells to EGFR-Tyrosine Kinase Inhibitor Erlotinib.
Raimbourg J, Joalland MP, Cabart M, de Plater L, Bouquet F, Savina A, Decaudin D, Bennouna J, Vallette FM, Lalier L
Molecular cancer therapeutics 2017 Aug;16(8):1634-1644
Molecular cancer therapeutics 2017 Aug;16(8):1634-1644
Monocyte Subsets Coregulate Inflammatory Responses by Integrated Signaling through TNF and IL-6 at the Endothelial Cell Interface.
Chimen M, Yates CM, McGettrick HM, Ward LS, Harrison MJ, Apta B, Dib LH, Imhof BA, Harrison P, Nash GB, Rainger GE
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2834-2843
Journal of immunology (Baltimore, Md. : 1950) 2017 Apr 1;198(7):2834-2843
Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium.
Munir H, Luu NT, Clarke LS, Nash GB, McGettrick HM
PloS one 2016;11(5):e0155161
PloS one 2016;11(5):e0155161
CXCL8 and CCL20 Enhance Osteoclastogenesis via Modulation of Cytokine Production by Human Primary Osteoblasts.
Pathak JL, Bakker AD, Verschueren P, Lems WF, Luyten FP, Klein-Nulend J, Bravenboer N
PloS one 2015;10(6):e0131041
PloS one 2015;10(6):e0131041
Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
Mar AC, Chu CH, Lee HJ, Chien CW, Cheng JJ, Yang SH, Jiang JK, Lee TC
The Journal of biological chemistry 2015 Sep 4;290(36):22212-24
The Journal of biological chemistry 2015 Sep 4;290(36):22212-24
Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S
Cancer research 2015 Jul 1;75(13):2629-40
Cancer research 2015 Jul 1;75(13):2629-40
Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
Voltolini C, Battersby S, Novembri R, Torricelli M, Severi FM, Petraglia F, Norman JE
Endocrinology 2015 Feb;156(2):670-9
Endocrinology 2015 Feb;156(2):670-9
Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2452-64
The Journal of neuroscience : the official journal of the Society for Neuroscience 2015 Feb 11;35(6):2452-64
Epstein-Barr virus infection induces indoleamine 2,3-dioxygenase expression in human monocyte-derived macrophages through p38/mitogen-activated protein kinase and NF-κB pathways: impairment in T cell functions.
Liu WL, Lin YH, Xiao H, Xing S, Chen H, Chi PD, Zhang G
Journal of virology 2014 Jun;88(12):6660-71
Journal of virology 2014 Jun;88(12):6660-71
Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
Kuravi SJ, McGettrick HM, Satchell SC, Saleem MA, Harper L, Williams JM, Rainger GE, Savage CO
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):234-43
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 1;193(1):234-43
Digital microfluidic assay for protein detection.
Mok J, Mindrinos MN, Davis RW, Javanmard M
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2110-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2110-5
Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner.
Mace TA, Ameen Z, Collins A, Wojcik S, Mair M, Young GS, Fuchs JR, Eubank TD, Frankel WL, Bekaii-Saab T, Bloomston M, Lesinski GB
Cancer research 2013 May 15;73(10):3007-18
Cancer research 2013 May 15;73(10):3007-18
Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
Todd J, Simpson P, Estis J, Torres V, Wub AH
Cytokine 2013 Dec;64(3):660-5
Cytokine 2013 Dec;64(3):660-5
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Nicolaidou V, Wong MM, Redpath AN, Ersek A, Baban DF, Williams LM, Cope AP, Horwood NJ
PloS one 2012;7(7):e39871
PloS one 2012;7(7):e39871
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
Hong YS, Moon SJ, Joo YB, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Park SH, Kim HY, Min JK
Journal of Korean medical science 2011 Sep;26(9):1132-9
Journal of Korean medical science 2011 Sep;26(9):1132-9
Protection from RNA and DNA viruses by IL-32.
Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Apr 1;186(7):4110-8
Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
Tso GH, Law HK, Tu W, Chan GC, Lau YL
Stem cells (Dayton, Ohio) 2010 May;28(5):939-54
Stem cells (Dayton, Ohio) 2010 May;28(5):939-54
TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
Aliahmadi E, Gramlich R, Grützkau A, Hitzler M, Krüger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
European journal of immunology 2009 May;39(5):1221-30
European journal of immunology 2009 May;39(5):1221-30
Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
Hu H, Kwun J, Aizenstein BD, Knechtle SJ
Transplantation 2009 Jun 27;87(12):1814-20
Transplantation 2009 Jun 27;87(12):1814-20
Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
AIDS research and human retroviruses 2008 May;24(5):667-71
AIDS research and human retroviruses 2008 May;24(5):667-71
Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
Kurago ZB, Lam-ubol A, Stetsenko A, De La Mater C, Chen Y, Dawson DV
Head and neck pathology 2008 Mar;2(1):1-12
Head and neck pathology 2008 Mar;2(1):1-12
C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome.
Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7368-75
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7368-75
Endogenous IL-32 controls cytokine and HIV-1 production.
Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Journal of immunology (Baltimore, Md. : 1950) 2008 Jul 1;181(1):557-65
Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
Liotta F, Angeli R, Cosmi L, Filì L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89
Stem cells (Dayton, Ohio) 2008 Jan;26(1):279-89
Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
Osei-Kumah A, Smith R, Clifton VL
Cytokine 2008 Aug;43(2):187-93
Cytokine 2008 Aug;43(2):187-93
Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2772-80
Journal of immunology (Baltimore, Md. : 1950) 2008 Aug 15;181(4):2772-80
Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2007 Oct 10;25(29):4542-9
Journal of clinical oncology : official journal of the American Society of Clinical Oncology 2007 Oct 10;25(29):4542-9
Ultrasensitive flow-based immunoassays using single-molecule counting.
Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
Clinical chemistry 2007 Nov;53(11):1990-5
Clinical chemistry 2007 Nov;53(11):1990-5
Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
Arnold R, Neumann M, König W
Immunology 2007 May;121(1):71-81
Immunology 2007 May;121(1):71-81
Th2 cytokine response in Major Depressive Disorder patients before treatment.
Pavón L, Sandoval-López G, Eugenia Hernández M, Loría F, Estrada I, Pérez M, Moreno J, Avila U, Leff P, Antón B, Heinze G
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Journal of neuroimmunology 2006 Mar;172(1-2):156-65
Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Arthritis and rheumatism 2006 Jul;54(7):2096-108
Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
Acta obstetricia et gynecologica Scandinavica 2005 Jun;84(6):551-7
Acta obstetricia et gynecologica Scandinavica 2005 Jun;84(6):551-7
Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
Phillips TM
Electrophoresis 2004 Jun;25(10-11):1652-9
Electrophoresis 2004 Jun;25(10-11):1652-9
Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
Copeland S, Siddiqui J, Remick D
Journal of immunological methods 2004 Jan;284(1-2):99-106
Journal of immunological methods 2004 Jan;284(1-2):99-106
trans fatty acids and systemic inflammation in heart failure.
Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
The American journal of clinical nutrition 2004 Dec;80(6):1521-5
Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation.
McLoughlin RM, Witowski J, Robson RL, Wilkinson TS, Hurst SM, Williams AS, Williams JD, Rose-John S, Jones SA, Topley N
The Journal of clinical investigation 2003 Aug;112(4):598-607
The Journal of clinical investigation 2003 Aug;112(4):598-607
Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5244-51
Journal of immunology (Baltimore, Md. : 1950) 2002 Nov 1;169(9):5244-51
The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
Arthritis and rheumatism 2001 Oct;44(10):2392-401
Arthritis and rheumatism 2001 Oct;44(10):2392-401
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Recombinant Human IL-6 by Western Blot. Western blot shows 25 ng of Recombinant Human IL-6 (Catalog # 206-IL), Recombinant Mouse IL-6 (Catalog # 406-ML) and Recombinant Rat IL-6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/ Primate IL-6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 in Human Skin. IL-6 was detected in immersion fixed frozen sections of hyperplastic human skin using Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Human IL-6 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (Catalog # MAB206). The ND50 is typically 0.05-0.15 µg/mL.
