Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF561 - Provider product page
- Provider
- R&D Systems
- Product name
- Cotton Rat IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects cotton rat IL-6 in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse IL-6 and recombinant rat IL-6 is observed and less than 5% cross-reactivity with recombinant porcine IL-6 and recombinant human IL-6 is observed.
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAL18819
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Synergistic induction of interferon α through TLR-3 and TLR-9 agonists identifies CD21 as interferon α receptor for the B cell response.
Kim D, Niewiesk S
PLoS pathogens 2013 Mar;9(3):e1003233
PLoS pathogens 2013 Mar;9(3):e1003233
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Cotton Rat IL-6 Antibody. Recombinant Cotton Rat IL-6 (Catalog # 561-R6) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Cotton Rat IL-6 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF561). The ND50 is typically 0.03-0.12 µg/mL.