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- Antibody Data
- Antigen structure
- References [4]
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- Western blot [1]
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- Product number
- PAB16919 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB16919, RRID:AB_10696391
- Product name
- IL6 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against full length recombinant IL6.
- Storage
- Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.
Submitted references Expression of TGF-beta isoforms by Thy-1+ and Thy-1- pulmonary fibroblast subsets: evidence for TGF-beta as a regulator of IL-1-dependent stimulation of IL-6.
Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity.
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity.
Silvera MR, Sempowski GD, Phipps RP
Lymphokine and cytokine research 1994 Oct;13(5):277-85
Lymphokine and cytokine research 1994 Oct;13(5):277-85
Enhancement of interleukin 6 cytostatic effect on human breast carcinoma cells by soluble IL-6 receptor from urine and reversion by monoclonal antibody.
Novick D, Shulman LM, Chen L, Revel M
Cytokine 1992 Jan;4(1):6-11
Cytokine 1992 Jan;4(1):6-11
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity.
Novick D, Eshhar Z, Revel M, Mory Y
Hybridoma 1989 Oct;8(5):561-7
Hybridoma 1989 Oct;8(5):561-7
Monoclonal antibodies for affinity purification of IL-6/IFN- beta 2 and for neutralization of HGF activity.
Novick D, Eshhar Z, Revel M, Mory Y
Hybridoma 1989 Oct;8(5):561-7
Hybridoma 1989 Oct;8(5):561-7
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot using IL6 polyclonal antibody (Cat # PAB16919) shows detection of a band ~24 KDa in size corresponding to recombinant human IL6 (arrowhead). Molecular weight markers are also shown. After transfer, the membrane was blocked overnight with 1% BSA in TTBS followed by reaction with primary antibody at a 1 : 500 dilution. Detection occurred using HRP conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer and FemtoMax™ Chemiluminescent substrate. Image was captured using VersaDoc MP 4000 imaging system (Bio-Rad).
