M00102-1

antibody from Boster Biological Technology

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00102-1

Provider

Boster Biological Technology

Product name

Anti-IL-6 Monoclonal Antibody

Antibody type

Monoclonal

Description

Mouse Monoclonal antibody for IL6 detection. Tested positive for ELISA,Flow Cytometry,Western Blot, in Human

Reactivity

Human

Host

Mouse

Vial size

100ug

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

Store IL 6 antibody at -20° C . For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Flow cytometry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

Anti-Human IL-6 Antibody - Flow CytometryHuman PBMCs were stimulated with 1ug/mL LPS and a transport inhibitor for 4-5 hours.Cells were then suspended in fixation buffer for 10-12 minutes and vortexed briefly.1mL of permeabilization buffer was added.0.5mg of Anti-Human IL-6 Antibody was added (0.125 ug/mL control antibody) and incubated in the dark for 30 minutes.1:100 of strep/PE was added and incubated for 30 minutes.LPS-stimulated samples were compared to unstimulated cells stained with strep/PE.

Additional image