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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX100525 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100525, RRID:AB_11178602
- Product name
- IL6 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
- Storage
- Keep as concentrated solution. Aliquot and store at -20?C or below. Avoid multiple freeze-thaw cycles.
Submitted references Altered Gene and Protein Expressions in Torn Rotator Cuff Tendon Tissues in Diabetic Patients.
Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients.
Chung SW, Choi BM, Kim JY, Lee YS, Yoon JP, Oh KS, Park KS
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 2017 Mar;33(3):518-526.e1
Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association 2017 Mar;33(3):518-526.e1
Immunoexpression of interleukin-6 in drug-induced gingival overgrowth patients.
Ganesh PR
Contemporary clinical dentistry 2016 Apr-Jun;7(2):140-5
Contemporary clinical dentistry 2016 Apr-Jun;7(2):140-5
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with IL6 antibody (GTX100525) diluted at 1:500.
- Protocol
- Protocol
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 μg of whole cell lysate) A: A549 B: H1299 C: HCT116 D: MCF-7 12% SDS PAGE GTX100525 diluted at 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IL6 antibody detects IL6 protein at cytoplasm by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IL6 protein stained by IL6 antibody (GTX100525) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC13 xenograft, using IL6(GTX100525) antibody at 1:500 dilution.
