AP52199PU-N

antibody from Acris Antibodies GmbH

Targeting: IL6 BSF2, HGF, HSF, IFNB2, IL-6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP52199PU-N

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP52199PU-N, RRID:AB_11148518

Product name

anti Interleukin-6 (IL6) (Center)

Antibody type

Polyclonal

Antigen

KLH conjugated synthetic peptide between 26-55 amino acids from the Central region of Human Interleukin-6

Reactivity

Human

Host

Rabbit

Vial size

0.4 ml

Concentration

lot specific

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western blot analysis of Interleukin-6 (IL6) Antibody (Center) Cat.-No AP52199PU-N in NCI-H460 cell line lysates (35ug/lane). This demonstrates the IL6 antibody detected the IL6 protein (arrow).

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue reacted with Interleukin-6 (IL6) Antibody (Center) Cat.-No AP52199PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.

Flow cytometry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Flow cytometric analysis of NCI-H460 cells using Interleukin-6 (IL6) Antibody (Center) Cat.-No AP52199PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.