Antibody data
- Antibody Data
- Antigen structure
- References [30]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AF-206-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-6 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human IL-6 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse IL-6 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P05231
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Osteoblasts are "educated" by crosstalk with metastatic breast cancer cells in the bone tumor microenvironment.
Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.
Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice.
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ.
SorLA in Interleukin-6 Signaling and Turnover.
Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway.
Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.
Inhibition of IL-6 expression in LNCaP prostate cancer cells by a combination of atorvastatin and celecoxib.
Digital microfluidic assay for protein detection.
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures: role of osteoblast matrix metalloproteinase (MMP)-13 in bone metastasis.
p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network.
Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Kolb AD, Shupp AB, Mukhopadhyay D, Marini FC, Bussard KM
Breast cancer research : BCR 2019 Feb 27;21(1):31
Breast cancer research : BCR 2019 Feb 27;21(1):31
Improved efficiency of in situ protein analysis by proximity ligation using UnFold probes.
Klaesson A, Grannas K, Ebai T, Heldin J, Koos B, Leino M, Raykova D, Oelrich J, Arngården L, Söderberg O, Landegren U
Scientific reports 2018 Mar 29;8(1):5400
Scientific reports 2018 Mar 29;8(1):5400
Human umbilical cord-derived mesenchymal stem cells direct macrophage polarization to alleviate pancreatic islets dysfunction in type 2 diabetic mice.
Yin Y, Hao H, Cheng Y, Zang L, Liu J, Gao J, Xue J, Xie Z, Zhang Q, Han W, Mu Y
Cell death & disease 2018 Jul 9;9(7):760
Cell death & disease 2018 Jul 9;9(7):760
Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies.
Jalili R, Horecka J, Swartz JR, Davis RW, Persson HHJ
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Proceedings of the National Academy of Sciences of the United States of America 2018 Jan 30;115(5):E925-E933
Pathomimetic avatars reveal divergent roles of microenvironment in invasive transition of ductal carcinoma in situ.
Sameni M, Cavallo-Medved D, Franco OE, Chalasani A, Ji K, Aggarwal N, Anbalagan A, Chen X, Mattingly RR, Hayward SW, Sloane BF
Breast cancer research : BCR 2017 May 15;19(1):56
Breast cancer research : BCR 2017 May 15;19(1):56
SorLA in Interleukin-6 Signaling and Turnover.
Larsen JV, Petersen CM
Molecular and cellular biology 2017 Jun 1;37(11)
Molecular and cellular biology 2017 Jun 1;37(11)
Adipose Stromal Cells from Visceral and Subcutaneous Fat Facilitate Migration of Ovarian Cancer Cells via IL-6/JAK2/STAT3 Pathway.
Kim B, Kim HS, Kim S, Haegeman G, Tsang BK, Dhanasekaran DN, Song YS
Cancer research and treatment : official journal of Korean Cancer Association 2017 Apr;49(2):338-349
Cancer research and treatment : official journal of Korean Cancer Association 2017 Apr;49(2):338-349
Combined CSL and p53 downregulation promotes cancer-associated fibroblast activation.
Procopio MG, Laszlo C, Al Labban D, Kim DE, Bordignon P, Jo SH, Goruppi S, Menietti E, Ostano P, Ala U, Provero P, Hoetzenecker W, Neel V, Kilarski WW, Swartz MA, Brisken C, Lefort K, Dotto GP
Nature cell biology 2015 Sep;17(9):1193-204
Nature cell biology 2015 Sep;17(9):1193-204
Il-6 signaling between ductal carcinoma in situ cells and carcinoma-associated fibroblasts mediates tumor cell growth and migration.
Osuala KO, Sameni M, Shah S, Aggarwal N, Simonait ML, Franco OE, Hong Y, Hayward SW, Behbod F, Mattingly RR, Sloane BF
BMC cancer 2015 Aug 13;15:584
BMC cancer 2015 Aug 13;15:584
Tumor-activated TCRγδ⁺ T cells from gastric cancer patients induce the antitumor immune response of TCRαβ⁺ T cells via their antigen-presenting cell-like effects.
Mao C, Mou X, Zhou Y, Yuan G, Xu C, Liu H, Zheng T, Tong J, Wang S, Chen D
Journal of immunology research 2014;2014:593562
Journal of immunology research 2014;2014:593562
Fucose-based PAMPs prime dendritic cells for follicular T helper cell polarization via DC-SIGN-dependent IL-27 production.
Gringhuis SI, Kaptein TM, Wevers BA, van der Vlist M, Klaver EJ, van Die I, Vriend LE, de Jong MA, Geijtenbeek TB
Nature communications 2014 Oct 3;5:5074
Nature communications 2014 Oct 3;5:5074
Inhibition of IL-6 expression in LNCaP prostate cancer cells by a combination of atorvastatin and celecoxib.
Wang H, Cui XX, Goodin S, Ding N, Van Doren J, Du Z, Huang MT, Liu Y, Cheng X, Dipaola RS, Conney AH, Zheng X
Oncology reports 2014 Feb;31(2):835-41
Oncology reports 2014 Feb;31(2):835-41
Digital microfluidic assay for protein detection.
Mok J, Mindrinos MN, Davis RW, Javanmard M
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2110-5
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 11;111(6):2110-5
Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Journal of immunological methods 2012 Apr 30;378(1-2):72-80
Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures: role of osteoblast matrix metalloproteinase (MMP)-13 in bone metastasis.
Morrison C, Mancini S, Cipollone J, Kappelhoff R, Roskelley C, Overall C
The Journal of biological chemistry 2011 Sep 30;286(39):34271-85
The Journal of biological chemistry 2011 Sep 30;286(39):34271-85
p38MAPK is a novel DNA damage response-independent regulator of the senescence-associated secretory phenotype.
Freund A, Patil CK, Campisi J
The EMBO journal 2011 Apr 20;30(8):1536-48
The EMBO journal 2011 Apr 20;30(8):1536-48
Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells.
Ara T, Song L, Shimada H, Keshelava N, Russell HV, Metelitsa LS, Groshen SG, Seeger RC, DeClerck YA
Cancer research 2009 Jan 1;69(1):329-37
Cancer research 2009 Jan 1;69(1):329-37
Epigallocatechin-3-gallate inhibits IL-6 synthesis and suppresses transsignaling by enhancing soluble gp130 production.
Ahmed S, Marotte H, Kwan K, Ruth JH, Campbell PL, Rabquer BJ, Pakozdi A, Koch AE
Proceedings of the National Academy of Sciences of the United States of America 2008 Sep 23;105(38):14692-7
Proceedings of the National Academy of Sciences of the United States of America 2008 Sep 23;105(38):14692-7
Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network.
Kuilman T, Michaloglou C, Vredeveld LC, Douma S, van Doorn R, Desmet CJ, Aarden LA, Mooi WJ, Peeper DS
Cell 2008 Jun 13;133(6):1019-31
Cell 2008 Jun 13;133(6):1019-31
Exploring human testicular peritubular cells: identification of secretory products and regulation by tumor necrosis factor-alpha.
Schell C, Albrecht M, Mayer C, Schwarzer JU, Frungieri MB, Mayerhofer A
Endocrinology 2008 Apr;149(4):1678-86
Endocrinology 2008 Apr;149(4):1678-86
T cell-dependent survival of CD20+ and CD20- plasma cells in human secondary lymphoid tissue.
Withers DR, Fiorini C, Fischer RT, Ettinger R, Lipsky PE, Grammer AC
Blood 2007 Jun 1;109(11):4856-64
Blood 2007 Jun 1;109(11):4856-64
Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.
Skibinski G, Elborn JS, Ennis M
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
American journal of physiology. Lung cellular and molecular physiology 2007 Jul;293(1):L69-76
Normalizing the bone marrow microenvironment with p38 inhibitor reduces multiple myeloma cell proliferation and adhesion and suppresses osteoclast formation.
Nguyen AN, Stebbins EG, Henson M, O'Young G, Choi SJ, Quon D, Damm D, Reddy M, Ma JY, Haghnazari E, Kapoun AM, Medicherla S, Protter A, Schreiner GF, Kurihara N, Anderson J, Roodman GD, Navas TA, Higgins LS
Experimental cell research 2006 Jun 10;312(10):1909-23
Experimental cell research 2006 Jun 10;312(10):1909-23
Interleukin-6 and vascular endothelial growth factor release by renal cell carcinoma cells impedes lymphocyte-dendritic cell cross-talk.
Cabillic F, Bouet-Toussaint F, Toutirais O, Rioux-Leclercq N, Fergelot P, de la Pintière CT, Genetet N, Patard JJ, Catros-Quemener V
Clinical and experimental immunology 2006 Dec;146(3):518-23
Clinical and experimental immunology 2006 Dec;146(3):518-23
Induction of interleukin-6 by hepatitis C virus core protein in hepatitis C-associated mixed cryoglobulinemia and B-cell non-Hodgkin's lymphoma.
Feldmann G, Nischalke HD, Nattermann J, Banas B, Berg T, Teschendorf C, Schmiegel W, Dührsen U, Halangk J, Iwan A, Sauerbruch T, Caselmann WH, Spengler U
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Aug 1;12(15):4491-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Aug 1;12(15):4491-8
Influence of eicosapentaenoic acid, an omega-3 fatty acid, on ultraviolet-B generation of prostaglandin-E2 and proinflammatory cytokines interleukin-1 beta, tumor necrosis factor-alpha, interleukin-6 and interleukin-8 in human skin in vivo.
Shahbakhti H, Watson RE, Azurdia RM, Ferreira CZ, Garmyn M, Rhodes LE
Photochemistry and photobiology 2004 Sep-Oct;80(2):231-5
Photochemistry and photobiology 2004 Sep-Oct;80(2):231-5
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
Journal of virology 2003 Feb;77(3):1727-37
Journal of virology 2003 Feb;77(3):1727-37
Arenavirus-mediated liver pathology: acute lymphocytic choriomeningitis virus infection of rhesus macaques is characterized by high-level interleukin-6 expression and hepatocyte proliferation.
Lukashevich IS, Tikhonov I, Rodas JD, Zapata JC, Yang Y, Djavani M, Salvato MS
Journal of virology 2003 Feb;77(3):1727-37
Journal of virology 2003 Feb;77(3):1727-37
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-6 by Western Blot. Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with PHA. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for IL-6 at approximately 20-22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- IL-6 in Human PBMCs. IL-6 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) treated with LPS and monensin using Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-6 and Neutralization by Human IL-6 Antibody. Recombinant Human IL-6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-206-NA). The ND50 is typically 15-45 ng/mL.