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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PAB4281 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB4281, RRID:AB_1575175
- Product name
- GGA1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of GGA1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references GGA1 acts as a spatial switch altering amyloid precursor protein trafficking and processing.
von Arnim CA, Spoelgen R, Peltan ID, Deng M, Courchesne S, Koker M, Matsui T, Kowa H, Lichtenthaler SF, Irizarry MC, Hyman BT
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Sep 27;26(39):9913-22
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Sep 27;26(39):9913-22
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The GGA1 polyclonal antibody (Cat # PAB4281) is used in Western blot to detect GGA1 in Jurkat cell lysate .
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The GGA1 polyclonal antibody (Cat # PAB4281) is used in Western blot to detect GGA1 in mouse kidney tissue lysate .
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- A : N2a cells cotransfected with APP770-V5 and GGA1-myc or empty vector were immunostained for APP (Alexa488; green), GGA1 (Cy5; blue), and the Golgi marker GM130 (Cy3; red).B : The same transfection was stained for the endosomal marker EEA1 (Cy3).C : To assess cell-surface localization, APP695-GFP and GGA1-myc or empty vector were cotransfected and then immunostained with an Ab to the APP ectodomain on ice without permeabilization (Cy3). Cells were then fixed, permeabilized, and stained for GGA1 (Cy5).(J. Neurosci. 2006 Sep 27;26(39):9913-9922)
- Validation comment
- Immunofluorescence
