Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- OAAB17058 - Provider product page
- Provider
- Aviva Systems Biology
- Product name
- MAPK3 antibody - N - terminal region
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Vial size
- 400ul
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Western blot analysis of ERK1 (arrow) using rabbit ERK1 (N-term) Pab (Cat. # OAAB17058). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAPK1 gene (Lane 2) (Origene Technologies). MAPK3 is strongly supported by BioGPS gene expression data to be expressed in HEK293
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Supportive validation
- Submitted by
- Aviva Systems Biology (provider)
- Main image
- Experimental details
- MAPK3 Antibody (N-term) (Cat. # OAAB17058) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram) .FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.