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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- Ab102223 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant ERK1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti HumanÊERK1 Antibody,ÊPrimary antibody,Êcould be used forÊWB, IHC, Flow, IF/ICC and so on.Application:WB:Ê1/2000 - 1/10000 Ê Ê ÊÊIHC:Ê1/100 - 1/200Flow: 1/200 - 1/1000IF/ICC:Ê1/50 - 1/200IP: 1/50FunctionInvolved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).Post-translationalDually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Dephosphorylated by PTPRJ at Tyr-204.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RR671
- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 2 mg/ml
- Storage
- Store at -20¡C for 2 years. Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ERK1 Antibody (Ab102223) - Western Blot All lanes: Recombinant ERK1 Antibody (Ab102223) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/30000 dilution Predicted band size: 43 kDa Observed band size: 40 kDa Exposure time: 27.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ERK1 Antibody (Ab102223) - IF/ICC Recombinant ERK1 Antibody (Ab102223) staining ERK1 in HeLa cells by IF/ICC (immunofluorescence/immunocytochemistry). Cells were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 10% goat serum for half an hour at room temperature. Samples were incubated with Recombinant ERK1 Antibody (Ab102223) (1/50) at 4°C. An Alexa Fluor® 488-conjugatedGoat Anti-Rabbit IgG polyclonal was used as the secondary antibody (1/500). DAPI (blue) was used as the nuclear counter stain. Control: PBS and secondary antibody, An Alexa Fluor® 488-conjugatedGoat Anti-Rabbit IgG (1/500).
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ERK1 Antibody (Ab102223) - IHC Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis Cervix cancer tissue labelling ERK1 with Recombinant ERK1 Antibody (Ab102223) at 1:200. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9.0.
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant ERK1 Antibody (Ab102223) - Flow Cytometry Overlay histogram showing HeLa cells stained with Recombinant ERK1 Antibody (Ab102223) (Red). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then incubated in the Recombinant ERK1 Antibody (Ab102223) (1/1000 dilution) in 1x PBS/1% BSA for 30 min at room temperature. The secondary antibody was used a Goat Anti-Rabbit Alexa Fluor® 488 (IgG H+L) at 1/2000 dilution for 20 min at room temperature. Unlabeled sample (Black) was used as a control.
