Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- AM09071PU-N - Provider product page
- Provider
- OriGene
- Product name
- FKBP52 (FKBP4) mouse monoclonal antibody, clone AT4D3, Purified
- Antibody type
- Monoclonal
- Description
- FKBP52 (FKBP4) mouse monoclonal antibody, clone AT4D3, Purified
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FKBP4
- Isotype
- IgG
- Antibody clone number
- AT4D3
- Vial size
- 100 µl
- Concentration
- 1.0 mg/ml
Submitted references Suppression of TDO-mediated tryptophan catabolism in glioblastoma cells by a steroid-responsive FKBP52-dependent pathway.
Ott M, Litzenburger UM, Rauschenbach KJ, Bunse L, Ochs K, Sahm F, Pusch S, Opitz CA, Blaes J, von Deimling A, Wick W, Platten M
Glia 2015 Jan;63(1):78-90
Glia 2015 Jan;63(1):78-90
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis: Cell lysates of HeLa (40 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human FKBP4 (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemistry: Paraffin embedded sections of Seminoma (Germ cell tumor) tissue were incubated with anti-human FKBP4 (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and Diaminobenzidine (DAB) was used for detection.
- Validation comment
- IHC