Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M01184 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PIM1 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for PIM1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IF, IHC, ICC, WB. Reactive species: Human, Mouse PIM1 information: Molecular Weight: 45412 MW; Subcellular Localization: Isoform 2: Cytoplasm. Nucleus; Tissue Specificity: Expressed primarily in cells of the hematopoietic and germline lineages. Isoform 1 and isoform 2 are both expressed in prostate cancer cell lines.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Antibody clone number
- ECB-16
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Immunohistochemical analysis of paraffin-embedded human prostate carcinoma, using PIM1 Antibody (M01184)PIM1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PIM1 Antibody (M01184)overnight at 4
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