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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF2330 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse Osteoactivin/GPNMB Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Osteoactivin/GPNMB in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human (rh) Osteoactivin, rhSyndecan-4, and recombinant mouse Syndecan-4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q8BVA0- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution.
Glycoprotein NMB: a novel Alzheimer's disease associated marker expressed in a subset of activated microglia.
Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes.
Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
Tol MJ, van der Lienden MJC, Gabriel TL, Hagen JJ, Scheij S, Veenendaal T, Klumperman J, Donker-Koopman WE, Verhoeven AJ, Overkleeft H, Aerts JM, Argmann CA, van Eijk M
Autophagy 2018;14(3):437-449
Autophagy 2018;14(3):437-449
Glycoprotein NMB: a novel Alzheimer's disease associated marker expressed in a subset of activated microglia.
Hüttenrauch M, Ogorek I, Klafki H, Otto M, Stadelmann C, Weggen S, Wiltfang J, Wirths O
Acta neuropathologica communications 2018 Oct 19;6(1):108
Acta neuropathologica communications 2018 Oct 19;6(1):108
Gpnmb Is a Potential Marker for the Visceral Pathology in Niemann-Pick Type C Disease.
Marques AR, Gabriel TL, Aten J, van Roomen CP, Ottenhoff R, Claessen N, Alfonso P, Irún P, Giraldo P, Aerts JM, van Eijk M
PloS one 2016;11(1):e0147208
PloS one 2016;11(1):e0147208
Glycoprotein nonmetastatic melanoma protein B extracellular fragment shows neuroprotective effects and activates the PI3K/Akt and MEK/ERK pathways via the Na+/K+-ATPase.
Ono Y, Tsuruma K, Takata M, Shimazawa M, Hara H
Scientific reports 2016 Mar 18;6:23241
Scientific reports 2016 Mar 18;6:23241
A role for Na+,K+-ATPase α1 in regulating Rab27a localisation on melanosomes.
Booth AE, Tarafder AK, Hume AN, Recchi C, Seabra MC
PloS one 2014;9(7):e102851
PloS one 2014;9(7):e102851
Lysosomal stress in obese adipose tissue macrophages contributes to MITF-dependent Gpnmb induction.
Gabriel TL, Tol MJ, Ottenhof R, van Roomen C, Aten J, Claessen N, Hooibrink B, de Weijer B, Serlie MJ, Argmann C, van Elsenburg L, Aerts JM, van Eijk M
Diabetes 2014 Oct;63(10):3310-23
Diabetes 2014 Oct;63(10):3310-23
Dependency of intraocular pressure elevation and glaucomatous changes in DBA/2J and DBA/2J-Rj mice.
Scholz M, Buder T, Seeber S, Adamek E, Becker CM, Lütjen-Drecoll E
Investigative ophthalmology & visual science 2008 Feb;49(2):613-21
Investigative ophthalmology & visual science 2008 Feb;49(2):613-21
Osteoactivin is a novel osteoclastic protein and plays a key role in osteoclast differentiation and activity.
Sheng MH, Wergedal JE, Mohan S, Lau KH
FEBS letters 2008 Apr 30;582(10):1451-8
FEBS letters 2008 Apr 30;582(10):1451-8
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Adhesion Mediated by Osteoactivin/GPNMB and Neutralization by Mouse Osteoactivin/GPNMB Antibody. Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (Catalog # 2330-AC), immobilized onto a microplate previously coated with Goat Anti-Mouse IgG Fc (Catalog # G-202-C), supports the adhesion of the SVEC4-10 mouse vascular endothelial cell line in a dose-dependent manner (orange line), as measured by endogenous cellular lysosomal acid phosphatase activity. Adhesion elicited by Recombinant Mouse Osteoactivin/GPNMB Fc Chimera (0.25 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse Osteoactivin/ GPNMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2330). The ND50 is typically 0.2-0.8 µg/mL.
