TA336465

antibody from OriGene

Targeting: ARHGDIB GDIA2, GDID4, Ly-GDI, RAP1GN1, RhoGDI2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA336465

Provider

OriGene

Product name

Mouse Monoclonal D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015)

Antibody type

Monoclonal

Description

Mouse Monoclonal D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015)

Host

Mouse

Conjugate

Unconjugated

Epitope

ARHGDIB

Isotype

IgG

Antibody clone number

97A1015

Vial size

100 µg

Concentration

0.5 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015) [TA336465] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (TA336465, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and t

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot: D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015) [TA336465] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and TA336465 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for di

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015) [TA336465] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow Cytometry: D4-GDI/RhoGDI2 (cleavage specific) Antibody (97A1015) [TA336465] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the (0.5 ug

Validation comment

FC