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LearnNB100-56556
antibody from Novus Biologicals
Targeting: ARHGDIB
GDIA2, GDID4, Ly-GDI, RAP1GN1, RhoGDI2
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-56556 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56556, RRID:AB_838086
- Product name
- Mouse Monoclonal D4-GDI/RhoGDI2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. A 28 kDa band is observed.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Autoantibodies specific to D4GDI modulate Rho GTPase mediated cytoskeleton remodeling and induce autophagy in T lymphocytes.
Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection of human mast cells.
Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer.
Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer.
Barbati C, Alessandri C, Vomero M, Vona R, Colasanti T, Vacirca D, Camerini S, Crescenzi M, Pendolino M, Truglia S, Conti F, Garofalo T, Sorice M, Pierdominici M, Valesini G, Malorni W, Ortona E
Journal of autoimmunity 2015 Apr;58:78-89
Journal of autoimmunity 2015 Apr;58:78-89
Dramatic caspase-dependent apoptosis in antibody-enhanced dengue virus infection of human mast cells.
Brown MG, Huang YY, Marshall JS, King CA, Hoskin DW, Anderson R
Journal of leukocyte biology 2009 Jan;85(1):71-80
Journal of leukocyte biology 2009 Jan;85(1):71-80
Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer.
Lee SH, Lee JW, Kim HS, Kim SY, Park WS, Kim SH, Lee JY, Yoo NJ
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 May;111(5):586-90
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 May;111(5):586-90
Immunohistochemical analysis of Omi/HtrA2 expression in stomach cancer.
Lee SH, Lee JW, Kim HS, Kim SY, Park WS, Kim SH, Lee JY, Yoo NJ
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 May;111(5):586-90
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica 2003 May;111(5):586-90
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Analysis of D4GDI in Jurkat cells using NB100-56556 at 2 ug/ml and D4-GDI (cleavage specific) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 detects both the full-length and cleaved forms of D4GDI while D4-GDI (cleavage specific) specifically detects only the cleaved form of the protein.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - Western blot analysis of NB100-56556 in whole Jurkat cell lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: D4-GDI/RhoGDI2 Antibody (10D774) [NB100-56556] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the NB100-56725 or NB100-56556 D4-GDI antibodies (0.5 ug antibody/test/1 million cells) and Intracellular Staining Flow Assay Kit. A: NB100-56725 which recognizes only cleaved D4-GDI. B: NB100-56556 which recognizes both full-length and cleaved D4-GDI.
