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LearnNB100-56725
antibody from Novus Biologicals
Targeting: ARHGDIB
GDIA2, GDID4, Ly-GDI, RAP1GN1, RhoGDI2
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [20]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- NB100-56725 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56725, RRID:AB_838088
- Product name
- Mouse Monoclonal D4-GDI/RhoGDI2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. In anti-Fas treated Jurkat, an approx. 23 kDa band should be observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Proteomic analyses of age related changes in A.BY/SnJ mouse hearts.
Differential regulation of caspase-9 by ionizing radiation- and UV-induced apoptotic pathways in thymic cells.
Biphasic expression of RhoGDI2 in the progression of breast cancer and its negative relation with lymph node metastasis.
A variant of estrogen receptor-{alpha}, hER-{alpha}36: transduction of estrogen- and antiestrogen-dependent membrane-initiated mitogenic signaling.
Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL.
Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL.
Pseudomonas aeruginosa exotoxin A induces human mast cell apoptosis by a caspase-8 and -3-dependent mechanism.
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species.
Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids.
Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids.
Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade.
RhoGDI2 is an invasion and metastasis suppressor gene in human cancer.
Interference with PDK1-Akt survival signaling pathway by UCN-01 (7-hydroxystaurosporine).
Epothilone B analogue (BMS-247550)-mediated cytotoxicity through induction of Bax conformational change in human breast cancer cells.
Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change.
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Nishtala K, Phong TQ, Steil L, Sauter M, Salazar MG, Kandolf R, Felix SB, Völker U, Klingel K, Hammer E
Proteome science 2013;11:29
Proteome science 2013;11:29
Differential regulation of caspase-9 by ionizing radiation- and UV-induced apoptotic pathways in thymic cells.
Okamoto M, Koga S, Tatsuka M
Mutation research 2010 Jun 1;688(1-2):78-87
Mutation research 2010 Jun 1;688(1-2):78-87
Biphasic expression of RhoGDI2 in the progression of breast cancer and its negative relation with lymph node metastasis.
Hu LD, Zou HF, Zhan SX, Cao KM
Oncology reports 2007 Jun;17(6):1383-9
Oncology reports 2007 Jun;17(6):1383-9
A variant of estrogen receptor-{alpha}, hER-{alpha}36: transduction of estrogen- and antiestrogen-dependent membrane-initiated mitogenic signaling.
Wang Z, Zhang X, Shen P, Loggie BW, Chang Y, Deuel TF
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 13;103(24):9063-8
Proceedings of the National Academy of Sciences of the United States of America 2006 Jun 13;103(24):9063-8
Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL.
Jenkins CE, Swiatoniowski A, Power MR, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):8000-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):8000-7
Pseudomonas aeruginosa-induced human mast cell apoptosis is associated with up-regulation of endogenous Bcl-xS and down-regulation of Bcl-xL.
Jenkins CE, Swiatoniowski A, Power MR, Lin TJ
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):8000-7
Journal of immunology (Baltimore, Md. : 1950) 2006 Dec 1;177(11):8000-7
Pseudomonas aeruginosa exotoxin A induces human mast cell apoptosis by a caspase-8 and -3-dependent mechanism.
Jenkins CE, Swiatoniowski A, Issekutz AC, Lin TJ
The Journal of biological chemistry 2004 Aug 27;279(35):37201-7
The Journal of biological chemistry 2004 Aug 27;279(35):37201-7
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Liu CY, Lee CF, Hong CH, Wei YH
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Mitochondrial DNA mutation and depletion increase the susceptibility of human cells to apoptosis.
Liu CY, Lee CF, Hong CH, Wei YH
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Annals of the New York Academy of Sciences 2004 Apr;1011:133-45
Hydroxylation at C4' or C6 is essential for apoptosis-inducing activity of flavanone through activation of the caspase-3 cascade and production of reactive oxygen species.
Ko CH, Shen SC, Chen YC
Free radical biology & medicine 2004 Apr 1;36(7):897-910
Free radical biology & medicine 2004 Apr 1;36(7):897-910
Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids.
Chen YC, Shen SC, Lin HY
Biochemical pharmacology 2003 Oct 1;66(7):1139-50
Biochemical pharmacology 2003 Oct 1;66(7):1139-50
Rutinoside at C7 attenuates the apoptosis-inducing activity of flavonoids.
Chen YC, Shen SC, Lin HY
Biochemical pharmacology 2003 Oct 1;66(7):1139-50
Biochemical pharmacology 2003 Oct 1;66(7):1139-50
Differential apoptosis-inducing effect of quercetin and its glycosides in human promyeloleukemic HL-60 cells by alternative activation of the caspase 3 cascade.
Shen SC, Chen YC, Hsu FL, Lee WR
Journal of cellular biochemistry 2003 Aug 1;89(5):1044-55
Journal of cellular biochemistry 2003 Aug 1;89(5):1044-55
RhoGDI2 is an invasion and metastasis suppressor gene in human cancer.
Gildea JJ, Seraj MJ, Oxford G, Harding MA, Hampton GM, Moskaluk CA, Frierson HF, Conaway MR, Theodorescu D
Cancer research 2002 Nov 15;62(22):6418-23
Cancer research 2002 Nov 15;62(22):6418-23
Interference with PDK1-Akt survival signaling pathway by UCN-01 (7-hydroxystaurosporine).
Sato S, Fujita N, Tsuruo T
Oncogene 2002 Mar 7;21(11):1727-38
Oncogene 2002 Mar 7;21(11):1727-38
Epothilone B analogue (BMS-247550)-mediated cytotoxicity through induction of Bax conformational change in human breast cancer cells.
Yamaguchi H, Paranawithana SR, Lee MW, Huang Z, Bhalla KN, Wang HG
Cancer research 2002 Jan 15;62(2):466-71
Cancer research 2002 Jan 15;62(2):466-71
Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
Chen YC, Shen SC, Lee WR, Hsu FL, Lin HY, Ko CH, Tseng SW
Biochemical pharmacology 2002 Dec 15;64(12):1713-24
Biochemical pharmacology 2002 Dec 15;64(12):1713-24
Emodin induces apoptosis in human promyeloleukemic HL-60 cells accompanied by activation of caspase 3 cascade but independent of reactive oxygen species production.
Chen YC, Shen SC, Lee WR, Hsu FL, Lin HY, Ko CH, Tseng SW
Biochemical pharmacology 2002 Dec 15;64(12):1713-24
Biochemical pharmacology 2002 Dec 15;64(12):1713-24
The protein kinase PKB/Akt regulates cell survival and apoptosis by inhibiting Bax conformational change.
Yamaguchi H, Wang HG
Oncogene 2001 Nov 22;20(53):7779-86
Oncogene 2001 Nov 22;20(53):7779-86
Molecular cloning and characterization of Bif-1. A novel Src homology 3 domain-containing protein that associates with Bax.
Cuddeback SM, Yamaguchi H, Komatsu K, Miyashita T, Yamada M, Wu C, Singh S, Wang HG
The Journal of biological chemistry 2001 Jun 8;276(23):20559-65
The Journal of biological chemistry 2001 Jun 8;276(23):20559-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Western blot analysis of D4GDI in Jurkat cells using NB100-56556 (IMG-396A) at 2 ug/ml and NB100-56725 (IMG-160A) at 0.1 ug/ml. Cells were treated with 2 uM staurosporine for different time periods. NB100-56556 (IMG-396A) detects both the full-length and cleaved forms of D4GDI. While (NB100-56725) IMG-160A specifically detects only the cleaved form of the protein.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - WB analysis: HL60 cells were treated for different time periods with 10 ng/ml of anti-Fas mAb (NB100-56725, Clone DX2). Harvested cells were lysed and resolved by SDS-PAGE and transferred onto membrane. D4-GDI/RhoGDI2 cleavage was detected.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - analysis of D4GD1-cv in formalin-fixed, paraffin-embedded human breast tumor tissue using an isotype control (top) and this antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in untreated (left) and staurosporine-treated (right) Ramos cells using 0.5 ug of was used for this test.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - HL60 cells were left untreated (green) or treated with camptothecin to induce apoptosis (red). Cells were analyzed by intracellular flow cytometry using either the (0.5 ug antibody/test/1 million cells) and ((as seen in B). this is because (cells not undergoing apoptosis) and cleaved (apoptotic cells) forms of D4-GDI.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-56725. Staining of cleaved D4-GDI in 10^6 untreated (left) and 3 hour staurosporine-treated (right) Jurkat cells using 0.5 ug of this antibody was used for this test. Green histogram represents isotype control; red represents anti-D4-GDI antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): D4-GDI/RhoGDI2 Antibody (97A1015) - (cleavage specific) [NB100-56725] - Analysis using the FITC conjugate of NB100-56725. Staining of cleaved D4-GDI in 10^6 camptothecin-treated (red) and untreated (green) HL60 cells using 0.5 ug of NB100-56060. The shaded histogram represents cells without antibody.
