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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A300-998A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BIG1/ARFGEF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 µg) from RKO, LNCaP, HEK293T, Jurkat, and GaMG cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (Product # A300-998A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BIG1/ARFGEF1 by western blot. Samples: Whole cell lysate (25 µg) from NIH 3T3, TCMK-1, and CT26 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (Product # A300-998A lot 2) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse BIG1/ARFGEF1 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg) and mouse NIH3T3 (M; 50 µg) cells. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody A300-998A used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BIG1/ARFGEF1 by immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BIG1/ARFGEF1 (Cat. No. A300-998A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BIG1/ARFGEF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RKO cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-BIG1/ARFGEF1 antibody (Product # A300-998A lot 2) used for IP at 6 µg per reaction. BIG1/ARFGEF1 was also immunoprecipitated by a previous lot of this antibody (A300-998A lot 1) and a second antibody against a different epitope of BIG1/ARFGEF1 (Antibody 2). For blotting immunoprecipitated BIG1/ARFGEF1 (Product # A300-998A) was used at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
