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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX110167 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110167, RRID:AB_1949910
- Product name
- TCP1 epsilon antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1C1] (GTX110167) diluted at 1:6000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ug of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX110167 diluted at 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Molt-4 (GTX27912) 7.5% SDS PAGE GTX110167 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TCP1 epsilon antibody detects TCP1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5 % SDS-PAGE, and blotted with TCP1 epsilon antibody (GTX110167) diluted by 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with TCP1 epsilon antibody [N1C1] (GTX110167) diluted at 1:6000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using TCP-1 epsilon(GTX110167) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of TCP1 epsilon protein from 293T whole cell extracts using 5 £gg of TCP1 epsilon antibody [N1C1] (GTX110167).Western blot analysis was performed using TCP1 epsilon antibody [N1C1] (GTX110167).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TCP1 epsilon antibody [N1C1] detects TCP1 epsilon protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. TCP1 epsilon antibody [N1C1] (GTX110167) diluted at 1:500.
