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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- AF4070 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Sheep Polyclonal SOX15 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human SOX15 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) SOX3, rhSOX14, and rhSOX21 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references SOX15 is a candidate tumor suppressor in pancreatic cancer with a potential role in Wnt/β-catenin signaling.
Thu KL, Radulovich N, Becker-Santos DD, Pikor LA, Pusic A, Lockwood WW, Lam WL, Tsao MS
Oncogene 2014 Jan 16;33(3):279-88
Oncogene 2014 Jan 16;33(3):279-88
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Human SOX15 by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line and BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human SOX15 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4070) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SOX15 at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
