PA5-46452

antibody from Invitrogen Antibodies

Targeting: C1QTNF12 ADIPOLIN, C1QDC2, CTRP12, FAM132A, MGC105127

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-46452

Provider

Invitrogen Antibodies

Product name

FAM132A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide sequence: MRRWAWAAVV VLLGPQLVLL GGVGARREAQ RTQQPGQRAD PPNATASASS

Concentration

0.5 mg/mL

References

Submitted references

CTRP12 ameliorates atherosclerosis by promoting cholesterol efflux and inhibiting inflammatory response via the miR-155-5p/LXRα pathway.

Wang G, Chen JJ, Deng WY, Ren K, Yin SH, Yu XH
Cell death & disease 2021 Mar 10;12(3):254

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MCF7 cell lysate using an anti-FAM132A polyclonal antibody (Product # PA5-46452).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 1 Effects of CTRP12 on cholesterol efflux and lipid accumulation in macrophages. A - G THP-1 macrophages were pretreated with or without 50 ug/mL ox-LDL for 48 h, and then transduced with PBS, LV-NC, or LV-CTRP12 for 72 h. A CTRP12 expression was determined by western blot. B , C Measurement of intracellular TC, FC, CE, and TG concentrations. D Representative images of Oil red O staining (x200). Scale bar = 20 mum. E , F Representative fluorescent images of NBD-cholesterol burden (x200). Cholesterol efflux mediated by apoA-I and HDL was quantified in these groups. Scale bar = 20 mum. G Representative fluorescent images of Dil-ox-LDL uptake (x200). Scale bar = 20 mum. Data are expressed as the mean +- SD from three independent experiments. ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 7 CTRP12 inhibits atherosclerosis in apoE -/- mice. A - C Western diet-fed apoE -/- mice were injected via the tail vein with PBS, LV-NC, or LV-CTRP12 ( n = 15 in each group). A Analysis of CTRP12 expression in the aortas by western blot ( n = 10). B The plaques (green arrows) in the aortic arch under a stereoscopic microscope ( n = 10); C Cryosections of the aortic root were stained with HE, Oil Red O, or Masson, followed by quantification of lesion area, lipid accumulation and collagen contents using Image-Pro Plus 7.0 software ( n = 10). Scale bar = 100 mum. Data are represented as the mean +- SD. **** P < 0.0001; ns not significant.