PA5-90717

antibody from Invitrogen Antibodies

Targeting: GCAT KBL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-90717

Provider

Invitrogen Antibodies

Product name

GCAT Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: KAHKYRYRHL DMADLEAKLQ EAQKHRLRLV ATDGAFSMDG DIAPLQEICC LASRYGALVF MDECHATGFL GPTGRGTDEL LGVMDQVTII NSTLGKALGG ASGGYTTGPG PLVSLLRQRA RPYLFSNSLP PAVVGCASKA LDLLMGSNTI VQSMAAKTQR FRSKMEAAGF TISGASHPIC PVMLGDARLA SRMADDMLKR GIFVIGFSYP VVPKGKARIR VQISAVHSEE DIDRCVEAFV EVGRLHGALP; Positive Samples: Mouse eye; Cellular Location: Mitochondrion, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.67 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of mouse eye, using GCAT Polyclonal antibody (Product # PA5-90717) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 10s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of GCAT in extracts of mouse eye using GCAT Polyclonal Antibody (Product # PA5-90717) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of GCAT was performed in C6 cells using GCAT Polyclonal Antibody (Product # PA5-90717).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of GCAT in paraffin-embedded human colon carcinoma using GCAT Polyclonal Antibody (Product # PA5-90717) at a dilution of 1:200.