LS-B4289

antibody from LSBio

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B4289

Provider

LSBio

Product name

IHC-plus™ BUB1B / BubR1 Antibody (clone 2G5) LS-B4289

Antibody type

Monoclonal

Description

Purified from ascites by Protein A

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2G5

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

ELISA

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-BUB1B / BubR1 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-BUB1B / BubR1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Proximity ligation assay

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.