Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [2]
- Proximity ligation assay [3]
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Validation data
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- Product number
- LS-B4289 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ BUB1B / BubR1 Antibody (clone 2G5) LS-B4289
- Antibody type
- Monoclonal
- Description
- Purified from ascites by Protein A
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2G5
- Storage
- Store at -20°C. Aliquot to avoid freeze/thaw cycles.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- BUB1B monoclonal antibody (M02), clone 2G5 Western blot of BUB1B expression in HeLa NE.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody (M02), clone 2G5.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 rabbit purified polyclonal 1:1200 and anti-BUB1B mouse monoclonal antibody 1:50. Signals were detected by Duolink 30 Detection.