AM20433PU-N

antibody from OriGene

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

AM20433PU-N

Provider

OriGene

Product name

BubR1 (BUB1B) mouse monoclonal antibody, clone 2G5, Purified

Antibody type

Monoclonal

Description

BubR1 (BUB1B) mouse monoclonal antibody, clone 2G5, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

BUB1B

Isotype

IgG

Antibody clone number

2G5

Vial size

50 µg

Concentration

0.47 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of BUB1B expression in Hela NE using Monoclonal BUB1B antibody (clone 2G5).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western Blot analysis of BUB1B expression in transfected 293T cell line using Monoclonal BUB1B antibody (clone 2G5).

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

RNAi Knockdown (Antibody validated): Western blot analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Cat#H00000701-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with AM20433PU-N BUB1B antibody (clone 2G5). GAPDH (36.1 kDa) used as specificity and loading control.

Validation comment

WB

ELISA

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged BUB1B is approximately 0.03 ng/ml as a capture antibody.

Validation comment

ELISA

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with anti-CDC20 Rabbit purified polyclonal 1/1200 and anti-BUB1B Mouse monoclonal antibody 1/50. Signals were detected by Duolink? 30 Detection Kit 613(Red), and nuclei were counterstained with DAPI (blue). Each Red dot represents the detection of protein-protein interaction complex.

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunoperoxidase of monoclonal BUB1B antibody on Formalin-Fixed Paraffin-Embedded Human spleen at 3 ug/ml.

Validation comment

IHC