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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3263 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3263, RRID:AB_1571805
- Product name
- BUB1B polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BUB1B.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Characterization of MAD2B and other mitotic spindle checkpoint genes.
The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner.
Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.
Cahill DP, da Costa LT, Carson-Walter EB, Kinzler KW, Vogelstein B, Lengauer C
Genomics 1999 Jun 1;58(2):181-7
Genomics 1999 Jun 1;58(2):181-7
The mouse mitotic checkpoint gene bub1b, a novel bub1 family member, is expressed in a cell cycle-dependent manner.
Davenport JW, Fernandes ER, Harris LD, Neale GA, Goorha R
Genomics 1999 Jan 1;55(1):113-7
Genomics 1999 Jan 1;55(1):113-7
Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1.
Chan GK, Schaar BT, Yen TJ
The Journal of cell biology 1998 Oct 5;143(1):49-63
The Journal of cell biology 1998 Oct 5;143(1):49-63
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BUB1B (arrow) using rabbit BUB1B polyclonal antibody (Cat # PAB3263). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the BUB1B gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with BUB1B polyclonal antibody (Cat # PAB3263) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. HC = hepatocarcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
