TA500607

antibody from OriGene

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500607

Provider

OriGene

Proper citation

OriGene Cat#TA500607, RRID:AB_2274881

Product name

Anti-BUB1B (BubR1) mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)

Antibody type

Monoclonal

Description

Anti-BUB1B (BubR1) mouse monoclonal antibody, clone OTI6E5 (formerly 6E5)

Host

Mouse

Conjugate

Unconjugated

Epitope

BUB1B

Isotype

IgG

Antibody clone number

OTI6E5

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BUB1B (RC205844, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BUB1B.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-BUB1B monoclonal antibody.(1:200)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-BUB1B mouse monoclonal antibody (TA500607) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BUB1B(RC205844).

Validation comment

IF

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunofluorescent staining of HeLa cells using anti-BUB1B mouse monoclonal antibody (TA500607).

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Carcinoma of bladder tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of breast tissue using anti-BUB1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded colon tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of colon tissue using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-BUB1Bmouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500607, Dilution 1:50)

Validation comment

IHC

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either pCMV6-ENTRY BUB1B(RC205844)(Red) or empty vector control plasmid(Blue) were immunostained with anti-BUB1B mouse monoclonal(TA500607, Dilution 1:1,000), and then analyzed by flow cytometry.

Validation comment

FC