AMM01904G

antibody from Leading Biology

Targeting: BUB1B Bub1A, BUBR1, MAD3L, SSK1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Proximity ligation assay (Recommended by provider)

Antibody Data

Product number

AMM01904G

Provider

Leading Biology

Product name

BUB1B / BubR1 Antibody (clone 2G5)

Antibody type

Monoclonal

Description

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Reactivity

Human

Host

Mouse

Antibody clone number

2G5

Storage

Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.